| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CA3 |
| Uniprot No | P07451 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-260aa |
| 氨基酸序列 | MAKEWGYASHNGPDHWHELFPNAKGENQSPIELHTKDIRHDPSLQPWSVS YDGGSAKTILNNGKTCRVVFDDTYDRSMLRGGPLPGPYRLRQFHLHWGSS DDHGSEHTVDGVKYAAELHLVHWNPKYNTFKEALKQRDGIAVIGIFLKIG HENGEFQIFLDALDKIKTKGKEAPFTKFDPSCLFPACRDYWTYQGSFTTP PCEECIVWLLLKEPMTVSSDQMAKLRSLLSSAENEPPVPLVSNWRPPQPI NNRVVRASFKLEHHHHHH |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CA3重组蛋白的3篇代表性参考文献(内容为模拟示例，实际文献需通过数据库核实)：
1. **文献名称**：*Expression and purification of recombinant human carbonic anhydrase III in Escherichia coli*
**作者**：Smith J, et al.
**摘要**：该研究成功构建了人CA3基因的原核表达载体，优化了在大肠杆菌中的可溶性表达条件，并通过镍柱亲和层析获得高纯度蛋白，酶活性测定显示重组CA3具有碳酸酐酶活性。
2. **文献名称**：*Structural insights into the redox regulation of human CA3 by site-directed mutagenesis*
**作者**：Tanaka M, et al.
**摘要**：利用重组CA3蛋白进行晶体结构解析，发现其活性中心附近的半胱氨酸残基在氧化还原状态下的构象变化，揭示了CA3在抗氧化应激中的潜在分子机制。
3. **文献名称**：*Functional characterization of recombinant CA3 in skeletal muscle metabolism*
**作者**：Li X, et al.
**摘要**：通过体外实验证明重组CA3蛋白可调节肌细胞线粒体pH平衡，并影响脂肪酸氧化代谢通路，提示其在肌肉能量代谢疾病中的研究价值。
(注：以上为学术文献模拟示例，实际引用请以真实出版物为准。)
**Background of CA3 Recombinant Protein**
Carbonic anhydrase III (CA3), a member of the carbonic anhydrase enzyme family, is a zinc-binding metalloprotein primarily expressed in skeletal muscle and adipose tissue. Unlike other isoforms (e.g., CA1. CA2), CA3 exhibits lower catalytic activity in CO₂ hydration but plays distinct roles in cellular processes, including oxidative stress response, metabolism, and ion homeostasis. It is implicated in modulating redox balance by interacting with glutathione and thioredoxin systems, safeguarding cells against reactive oxygen species (ROS).
Recombinant CA3 protein is produced via genetic engineering, typically by inserting the CA3 gene into bacterial (e.g., *E. coli*) or mammalian expression systems, followed by purification. This approach ensures high-purity, functional protein for research. Its applications span structural studies (e.g., crystallography), enzyme kinetics, and exploring CA3's non-catalytic roles in muscle physiology and metabolic diseases.
CA3 dysregulation is linked to conditions like diabetes, obesity, and muscle disorders. For instance, reduced CA3 levels correlate with insulin resistance, while its overexpression may protect against oxidative damage in muscular dystrophy. Recombinant CA3 also serves as a biomarker in disease models, aiding therapeutic target discovery. Ongoing research focuses on its interplay with signaling pathways and potential as a diagnostic or therapeutic tool, highlighting its multifaceted role beyond traditional enzymatic functions.
In summary, recombinant CA3 protein is a vital tool for deciphering CA3's biological significance and translational potential in health and disease.
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