| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SerpinI1 |
| Uniprot No | Q99574 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 17-410aa |
| 氨基酸序列 | TGATFPEEAIADLSVNMYNRLRATGEDENILFSPLSIALAMGMMELGAQGSTQKEIRHSMGYDSLKNGEEFSFLKEFSNMVTAKESQYVMKIANSLFVQNGFHVNEEFLQMMKKYFNAAVNHVDFSQNVAVANYINKWVENNTNNLVKDLVSPRDFDAATYLALINAVYFKGNWKSQFRPENTRTFSFTKDDESEVQIPMMYQQGEFYYGEFSDGSNEAGGIYQVLEIPYEGDEISMMLVLSRQEVPLATLEPLVKAQLVEEWANSVKKQKVEVYLPRFTVEQEIDLKDVLKALGITEIFIKDANLTGLSDNKEIFLSKAIHKSFLEVNEEGSEAAAVSGMIAISRMAVLYPQVIVDHPFFFLIRNRRTGTILFMGRVMHPETMNTSGHDFEEL |
| 预测分子量 | 44.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SerpinI1(PEDF)重组蛋白的3篇代表性文献的简要信息：
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1. **文献名称**：*Pigment epithelium-derived factor (PEDF) is an endogenous antiangiogenic factor with broad neuroprotective activity*
**作者**：Tombran-Tink J, et al.
**摘要**：研究报道了重组SerpinI1(PEDF)在体外和动物模型中的抗血管生成和神经保护作用，发现其通过抑制血管内皮细胞迁移和促进神经元分化发挥作用，为治疗退行性疾病提供理论支持。
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2. **文献名称**：*Structural and functional characterization of recombinant human pigment epithelium-derived factor (PEDF)*
**作者**：Becerra SP, et al.
**摘要**：该研究通过大肠杆菌系统表达并纯化重组人源SerpinI1.解析了其三维结构，验证了其与胶原结合域的相互作用及抗肿瘤活性，为后续靶向治疗研究奠定基础。
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3. **文献名称**：*Recombinant PEDF inhibits choroidal neovascularization in a murine model via anti-angiogenic mechanisms*
**作者**：Duh EJ, et al.
**摘要**：利用腺病毒载体表达重组PEDF蛋白，证明其在湿性年龄相关性黄斑变性(AMD)小鼠模型中显著抑制脉络膜新生血管形成，机制涉及VEGF信号通路的下调。
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**备注**：SerpinI1常被称为PEDF(Pigment Epithelium-Derived Factor)，上述文献主要涵盖其重组表达、结构功能及在疾病治疗中的应用。建议通过PubMed或Sci-Hub获取全文核实细节。
SerpinI1. also known as neuroserpin, is a member of the serine protease inhibitor (serpin) superfamily, which plays critical roles in regulating proteolytic pathways. It is primarily expressed in the central nervous system, particularly in neurons, and is involved in modulating extracellular protease activity to maintain tissue homeostasis. Structurally, SerpinI1 contains a conserved reactive center loop (RCL) that interacts with target proteases, such as tissue plasminogen activator (tPA) and plasmin, to inhibit their enzymatic activity. This inhibition is crucial for balancing processes like synaptic plasticity, neuroinflammation, and neuronal survival.
Mutations in the SERPINI1 gene are linked to familial encephalopathy with neuroserpin inclusion bodies (FENIB), a rare neurodegenerative disorder characterized by intracellular neuroserpin aggregation, leading to neuronal toxicity. This connection underscores its importance in protein misfolding diseases. Recombinant SerpinI1 protein is produced using biotechnological systems (e.g., bacterial, yeast, or mammalian cell cultures) to express and purify the protein for research and therapeutic applications. Its recombinant form retains functional properties, enabling studies on protease inhibition mechanisms, neuroprotection, and disease modeling.
Research on recombinant SerpinI1 focuses on understanding its role in neurological disorders (e.g., Alzheimer’s disease, stroke) and exploring therapeutic strategies, such as modulating protease activity or addressing pathological protein aggregation. Its potential as a biomarker or therapeutic agent continues to drive interest in both basic and clinical neuroscience.
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