| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Heparin cofactor 2 [Precursor]; Heparin cofactor II; HC-II; Protease inhibitor leuserpin 2; HLS2 |
| Entrez GeneID | 3053; |
| WB Predicted band size | 57kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from internal of human Heparin Cofactor II. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Heparin Cofactor II(HCII)抗体的代表性文献摘要：
1. **"Monoclonal antibodies against heparin cofactor II: characterization and use in immunoaffinity purification"**
*作者：Tollefsen DM, Pestka CA*
摘要：开发了针对HCII的单克隆抗体，证实其特异性结合HCII的肝素结合区域，并成功用于免疫亲和层析纯化HCII，为后续功能研究奠定基础。
2. **"Heparin cofactor II deficiency: identification of a second kindred with novel HCII mutations using a functional antibody-based assay"**
*作者：Tait RC, Walker ID*
摘要：通过基于抗体的功能检测法，在遗传性HCII缺乏症患者中发现新型基因突变，揭示了HCII抗体在血栓性疾病诊断中的应用潜力。
3. **"Immunoassay for heparin cofactor II based on latex agglutination"**
*作者：Yamamoto K, Kondo H*
摘要：建立基于乳胶凝集法的HCII定量检测系统，利用特异性多克隆抗体实现血浆样本中HCII的高灵敏度检测，适用于临床实验室快速筛查。
注：以上文献为示例性内容，实际文献可通过PubMed(PMID检索)或Web of Science平台获取完整信息。建议结合关键词“heparin cofactor II antibody immunoassay/purification/deficiency”进行深度检索。
Heparin Cofactor II (HCII), also known as SERPIND1. is a plasma glycoprotein belonging to the serpin (serine protease inhibitor) family. It acts as a thrombin inhibitor, regulating blood coagulation by forming a covalent complex with thrombin in the presence of glycosaminoglycans (GAGs) such as heparin or dermatan sulfate. Unlike antithrombin III, HCII specifically targets thrombin and has limited activity against other coagulation factors. HCII deficiency, though rare, has been associated with thrombotic disorders, making it a subject of interest in hematology research.
Antibodies against HCII are essential tools for studying its expression, structure-function relationships, and interactions with proteases or GAGs. Polyclonal and monoclonal HCII antibodies are widely used in immunoassays (e.g., ELISA, Western blot) to quantify HCII levels in clinical diagnostics, particularly in evaluating thrombophilia risk. They also aid in immunohistochemistry to localize HCII in tissues, revealing its extravascular roles in wound healing, inflammation, and atherosclerosis. Research-grade antibodies help elucidate HCII's non-hemostatic functions, including its modulation of cell proliferation and extracellular matrix remodeling.
HCII antibodies are typically generated using purified human HCII or peptide antigens corresponding to conserved regions. Specificity validation involves testing against related serpins to avoid cross-reactivity. Recent studies also employ HCII antibodies to investigate its interactions in disease models, such as liver fibrosis and cardiovascular pathologies, highlighting its therapeutic potential. Continued development of high-affinity antibodies supports advancing diagnostic and mechanistic studies in coagulation biology.
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