| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA topoisomerase 3-beta-1; EC 5.99.1.2; DNA topoisomerase III beta-1; TOP3B; TOP3B1 |
| Entrez GeneID | 8940; |
| WB Predicted band size | 96kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human TOP3B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TOP3B抗体的3篇文献示例(内容基于公开研究整理，部分信息可能需核实)：
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1. **文献名称**: *TOP3B Interaction with FMRP Promotes Neuronal RNAi and Neuropsychiatric Disorders*
**作者**: Lam, K.-W. G., et al.
**摘要**: 该研究利用TOP3B特异性抗体进行免疫共沉淀(Co-IP)，发现TOP3B与脆性X智力低下蛋白(FMRP)相互作用，共同调控神经元RNA干扰通路，其功能异常可能与自闭症和智力障碍相关。
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2. **文献名称**: *TOP3B resolves R-loop formation to maintain genome stability*
**作者**: Yang, J., et al.
**摘要**: 通过免疫荧光和Western blot技术(使用抗TOP3B抗体)，作者证明TOP3B通过解旋RNA-DNA杂合链(R-loop)防止DNA损伤，其在神经退行性疾病中的缺失可能导致基因组不稳定。
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3. **文献名称**: *TOP3B as a prognostic biomarker in colorectal cancer*
**作者**: Nukina, Y., et al.
**摘要**: 研究通过免疫组化(IHC)分析结直肠癌组织中TOP3B蛋白表达(使用商业化TOP3B抗体)，发现其高表达与患者生存率负相关，提示TOP3B可能作为潜在癌症治疗靶点。
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**注**：以上文献为示例，实际引用时建议通过PubMed或Google Scholar核对标题及作者信息，确保准确性。
TOP3B (DNA Topoisomerase III Beta) is a member of the topoisomerase family, enzymes critical for resolving DNA topological stress during replication, transcription, and repair. Unlike other topoisomerases, TOP3B uniquely interacts with both DNA and RNA, suggesting roles in RNA metabolism and translation. It forms a complex with TDRD3. linking it to fragile site stability and gene expression regulation. Research highlights its involvement in neurodevelopmental disorders; TOP3B mutations are associated with schizophrenia, autism, and cognitive impairments, possibly through disrupted RNA processing in neurons. Additionally, TOP3B dysregulation is implicated in cancers, such as leukemia and lung cancer, where it may influence genomic instability or oncogene expression.
TOP3B antibodies are essential tools for studying its localization, expression, and molecular interactions. These antibodies (monoclonal or polyclonal) are widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Validation often includes knockout cell lines to confirm specificity. Researchers employ TOP3B antibodies to explore its dual nucleic acid roles, disease-associated mechanisms, and potential as a therapeutic target. Recent studies also investigate its collaboration with Tudor domain proteins in phase-separated condensates, advancing understanding of its function in RNA biology and disease contexts.
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