| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | T-box transcription factor TBX15; T-box protein 15; TBX15; TBX14; T-box transcription factor TBX18 |
| Entrez GeneID | 6913; |
| WB Predicted band size | 64kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human TBX15/18. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TBX15/18抗体的3篇模拟参考文献(示例格式，非真实文献)：
1. **《TBX18在心脏发育中的功能及其抗体特异性验证》**
- 作者：Smith A, et al.
- 摘要：研究通过生成兔源多克隆抗体，验证了TBX18在胚胎小鼠心脏祖细胞中的表达，证实抗体在免疫组化和Western blot中的特异性，揭示了TBX18对心脏传导系统发育的关键调控作用。
2. **《TBX15抗体在骨骼肌分化研究中的应用》**
- 作者：Chen L, et al.
- 摘要：该文献报道了一种小鼠单克隆抗体的开发，用于检测TBX15在成肌细胞分化中的动态表达，证实TBX15通过调控MyoD信号通路促进肌肉再生，抗体特异性经siRNA敲除实验验证。
3. **《Comparative analysis of TBX15 and TBX18 antibody staining patterns in embryonic tissues》**
- 作者：Wang Y, et al.
- 摘要：研究比较了两种商业化抗体(抗TBX15和抗TBX18)在小鼠胚胎不同组织中的染色结果，发现二者在肢体芽和神经嵴中具有互补表达模式，强调了抗体选择对发育生物学研究的重要性。
(注：以上为模拟示例，实际文献需通过PubMed/Google Scholar等平台检索。)
TBX15 and TBX18 are members of the T-box transcription factor family, which play critical roles in embryonic development, particularly in regulating cell fate determination, tissue differentiation, and organogenesis. TBX15 is implicated in the development of mesodermal derivatives, including skeletal and craniofacial structures, while TBX18 is essential for the formation of the posterior mesoderm-derived tissues, such as the ureteric bud in kidneys and specific components of the cardiac conduction system. Both genes are evolutionarily conserved and share structural homology, characterized by a DNA-binding T-domain that enables sequence-specific interactions with target gene promoters.
Antibodies targeting TBX15/18 are vital tools for studying their expression patterns, molecular functions, and regulatory mechanisms in developmental biology and disease models. These antibodies are commonly used in techniques like immunohistochemistry, Western blotting, and immunofluorescence to localize TBX15/18 proteins in tissues or cultured cells. Their specificity is validated through knockout controls or peptide-blocking assays to ensure accurate detection. Research applications include investigating congenital disorders (e.g., heart defects, skeletal malformations), cancer (where aberrant TBX15/18 expression may correlate with metastasis or proliferation), and regenerative medicine. Recent studies also explore their roles in cellular reprogramming and tissue homeostasis. Commercially available TBX15/18 antibodies are typically raised in rabbits or mice, with variations in clonality (monoclonal/polyclonal) and epitope targeting to accommodate diverse experimental needs.
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