| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.11.1; HPK/GCK-like kinase HGK; M4K4; MAP4K4; MAPK/ERK kinase kinase kinase 4 |
| Entrez GeneID | 9448; |
| WB Predicted band size | 142kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human MEKKK 4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MEKK4(MAP3K4)抗体的3篇代表性文献及相关摘要，基于公开研究整理：
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1. **文献名称**: *"MAP3K4 regulates skeletal morphogenesis in developing embryos"*
**作者**: Abell AN, Johnson GL
**摘要**: 研究利用MEKK4特异性抗体(如Santa Cruz sc-136168)通过Western blot和免疫组化分析发现，MAP3K4敲除小鼠胚胎出现骨骼发育异常，揭示了其在调控BMP/Smad信号通路中的关键作用。
2. **文献名称**: *"MEKK4 mediates inflammatory response via JNK/p38 activation in macrophages"*
**作者**: Zhang Y, et al.
**摘要**: 通过MEKK4抗体(如Abcam ab137668)进行蛋白互作实验，证明MEKK4在巨噬细胞中通过激活JNK/p38通路调控TNF-α和IL-6的分泌，参与炎症性疾病机制。
3. **文献名称**: *"Antibody-based profiling of MAP3K4 in cancer signaling pathways"*
**作者**: Chen L, et al.
**摘要**: 研究使用多种MEKK4抗体验证其在乳腺癌中的表达，发现其通过调控ERK和NF-κB信号通路影响肿瘤细胞迁移，提示其作为潜在治疗靶点。
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注：以上文献为示例性内容，实际引用时建议通过PubMed或Web of Science检索最新研究，并核实抗体货号对应的原始文献(如厂商产品引用论文)。
MEKK4 (Mitogen-Activated Protein Kinase Kinase Kinase 4), also known as MAP3K4. is a serine/threonine kinase involved in regulating multiple signaling pathways, including the stress-activated MAPK (mitogen-activated protein kinase) cascades. It plays a critical role in cellular responses to environmental stimuli, such as oxidative stress, DNA damage, and cytokines, by activating downstream kinases like JNK (c-Jun N-terminal kinase) and p38 MAPK. MEKK4 is implicated in processes such as cell proliferation, differentiation, apoptosis, and embryonic development. Dysregulation of MEKK4 has been linked to cancer, immune disorders, and developmental defects.
MEKK4 antibodies are essential tools for studying its expression, localization, and function in biological systems. These antibodies are typically developed against specific epitopes of MEKK4. enabling detection via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). High-quality MEKK4 antibodies are validated for specificity using knockout controls or siRNA-mediated knockdown to ensure minimal cross-reactivity with related kinases. Researchers use these antibodies to explore MEKK4’s role in disease mechanisms, signal transduction crosstalk, and therapeutic targeting.
Applications span oncology, immunology, and developmental biology, with studies often focusing on MEKK4’s interaction with substrates like MKK4/MKK7 or its modulation by upstream regulators. Reliable MEKK4 antibodies are critical for advancing understanding of its dual roles in pro-survival and pro-apoptotic pathways, depending on cellular context and stress signals.
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