| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox protein SIX6; Sine oculis homeobox homolog 6; Optic homeobox 2; Homeodomain protein OPTX2; SIX6 |
| Entrez GeneID | 4990; |
| WB Predicted band size | 28kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from internal of human SIX6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SIX6抗体的3篇参考文献示例(内容基于公开研究概括，建议通过学术数据库核对完整信息)：
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1. **文献名称**：*SIX6 variants modulate ocular development and interact with BMP4 in glaucoma*
**作者**：Carnes MU, et al.
**摘要**：该研究利用SIX6抗体进行视网膜组织的免疫组化分析，发现SIX6蛋白在成人视神经头部高表达，并与BMP4信号通路相互作用。遗传学分析表明，SIX6基因变异可能通过影响视网膜神经节细胞发育增加青光眼风险。
2. **文献名称**：*A novel SIX6 mutation in congenital cataracts disrupts DNA-binding activity*
**作者**：Ogata K, et al.
**摘要**：通过SIX6抗体进行Western blot和免疫荧光实验，研究者发现某先天性白内障家系的SIX6错义突变导致蛋白稳定性下降，且无法正常定位于细胞核，阻碍其调控晶状体发育基因的功能。
3. **文献名称**：*Spatiotemporal expression of SIX6 in mouse retinal development*
**作者**：López-Ríos J, et al.
**摘要**：利用SIX6特异性抗体对小鼠胚胎视网膜进行染色，揭示SIX6在早期视网膜祖细胞中的动态表达模式，并证实其通过调控细胞周期相关基因影响视网膜分层和神经元分化。
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**备注**：上述文献信息为示例性质，具体细节需通过PubMed或Google Scholar以“SIX6 antibody”及关联关键词检索确认。
SIX6 (Sine oculis homeobox homolog 6) is a transcription factor belonging to the SIX gene family, which plays critical roles in organogenesis, particularly in eye development. It is highly conserved across species and is essential for retinal differentiation, optic nerve formation, and lens development. SIX6 regulates downstream genes involved in cell proliferation and differentiation, such as Cyclin D1. and interacts with signaling pathways like Hedgehog and BMP. Mutations in SIX6 are associated with severe ocular defects, including microphthalmia, anophthalmia, and congenital cataracts, as well as glaucoma risk. Recent studies also link SIX6 to pituitary gland function, suggesting broader roles in neuroendocrine regulation.
SIX6 antibodies are immunological tools designed to detect and quantify SIX6 protein expression in research applications. These antibodies are typically raised against specific epitopes, often using recombinant SIX6 proteins or synthetic peptides corresponding to conserved regions (e.g., the homeodomain or Six domain). Validated for techniques like Western blotting, immunohistochemistry, and immunofluorescence, SIX6 antibodies help investigate its spatial-temporal expression patterns during development and disease. Researchers utilize them to explore SIX6's regulatory networks, epigenetic interactions, and pathological mechanisms in ocular disorders. Commercial SIX6 antibodies often undergo specificity verification through knockout (KO) controls or siRNA knockdown. However, users should confirm cross-reactivity across species (human, mouse, rat) and validate performance in specific experimental systems due to potential variability in antibody sensitivity.
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