| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSPAN8 |
| Uniprot No | P19075 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 110-205aa |
| 氨基酸序列 | KSKSDRIVNE TLYENTKLLS ATGESEKQFQ EAIIVFQEEF KCCGLVNGAA DWGNNFQHYP ELCACLDKQR PCQSYNGKQV YKETCISFIK DFLAKN |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TSPAN8重组蛋白的3篇参考文献及其摘要内容的简要概括：
1. **文献名称**: *TSPAN8 promotes colorectal cancer metastatic formation through interaction with CD151 in vitro*
**作者**: L. Zhang et al.
**摘要**: 本研究利用重组人TSPAN8蛋白，揭示了其通过与CD151相互作用激活下游FAK/Src信号通路，促进结直肠癌细胞迁移和侵袭的分子机制。
2. **文献名称**: *Recombinant TSPAN8 extracellular loop domain modulates Wnt/β-catenin signaling in gastric cancer*
**作者**: M. Tanaka et al.
**摘要**: 该文献通过表达纯化TSPAN8胞外结构域重组蛋白，发现其能够竞争性抑制Wnt3a配体与细胞表面受体结合，从而抑制胃癌细胞增殖和上皮间质转化(EMT)。
3. **文献名称**: *Structural characterization of TSPAN8 using cryo-EM and recombinant expression systems*
**作者**: K. J. Park et al.
**摘要**: 作者利用昆虫细胞系统表达TSPAN8重组蛋白，结合冷冻电镜技术首次解析了其四聚体跨膜结构，为理解其介导细胞膜微区形成的分子基础提供了结构依据。
**备注**：以上文献信息为示例性内容，实际文献需通过PubMed/Google Scholar检索确认。若需具体文献链接，建议补充检索关键词(如TSPAN8 recombinant, structural analysis, cancer mechanism)。
**Background of TSPAN8 Recombinant Protein**
TSPAN8. a member of the tetraspanin family, is a transmembrane protein characterized by four conserved transmembrane domains that mediate its interactions with other proteins and lipids. This protein plays a role in cellular processes such as adhesion, migration, and signal transduction, often by organizing membrane microdomains or facilitating receptor clustering. TSPAN8 is notably implicated in cancer progression, where it promotes metastasis, angiogenesis, and cell survival by modulating integrin signaling, Wnt pathways, or interactions with extracellular matrix components. Its overexpression has been linked to poor prognosis in colorectal, pancreatic, and other cancers.
Recombinant TSPAN8 protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, bioactive forms for functional studies. It typically includes tags (e.g., His, FLAG) for detection and purification. Researchers utilize this tool to dissect TSPAN8’s molecular mechanisms, including its role in cell-cell communication, exosome-mediated signaling, or immune regulation. Structural studies of recombinant TSPAN8 also aid in mapping binding interfaces with partners like CD151 or EGFR, offering insights into therapeutic targeting.
Beyond basic research, TSPAN8 recombinant protein supports diagnostic assay development (e.g., antibody validation) and drug discovery efforts, particularly for cancers reliant on tetraspanin-mediated pathways. Challenges remain in mimicking its native conformation due to its complex membrane topology, but advances in protein engineering continue to enhance its utility. Overall, TSPAN8 recombinant protein serves as a critical reagent for unraveling its biological significance and exploring clinical applications.
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