| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FZD6 |
| Uniprot No | O60353 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 71-181aa |
| 氨基酸序列 | PNIETFLCKAFVPTCIEQIHVVPPCRKLCEKVYSDCKKLIDTFGIRWPEE LECDRLQYCDETVPVTFDPHTEFLGPQKKTEQVQRDIGFWCPRHLKTSGG QGYKFLGIDQC |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FZD6重组蛋白的3篇参考文献及其简要摘要：
1. **"Structural basis of Wnt recognition by Frizzled"**
*Authors: J. Chen et al.*
摘要：该研究解析了FZD6重组蛋白与Wnt配体结合的晶体结构，揭示了FZD6胞外结构域如何特异性识别Wnt蛋白，为Wnt信号通路的激活机制提供了分子基础。
2. **"Expression and functional characterization of recombinant FZD6 in cancer stem cells"**
*Authors: M. Tanaka et al.*
摘要：研究通过哺乳动物细胞系统成功表达并纯化FZD6重组蛋白，发现其在癌症干细胞中调控β-catenin非依赖性信号通路，影响细胞迁移和侵袭能力。
3. **"FZD6-mediated Wnt signaling regulates planar cell polarity in vertebrates"**
*Authors: S. Wang et al.*
摘要：利用重组FZD6蛋白进行功能实验，证实其通过激活平面细胞极性(PCP)通路调控胚胎发育中的组织形态发生，突变体实验进一步揭示了关键结构域的功能差异。
*注：上述文献为示例，实际引用时需核对真实出版物信息。建议通过PubMed或Google Scholar以“FZD6 recombinant protein”为关键词检索最新研究。*
FZD6 (Frizzled class receptor 6) is a member of the Frizzled family of G protein-coupled receptors that primarily functions as a receptor for Wnt signaling molecules. As a key component of the Wnt/β-catenin and planar cell polarity (PCP) pathways, FZD6 plays critical roles in embryonic development, tissue homeostasis, and stem cell regulation. Structurally, it features a conserved extracellular cysteine-rich domain (CRD) for Wnt ligand binding, seven transmembrane helices, and an intracellular C-terminal tail involved in signal transduction.
Recombinant FZD6 protein is engineered to study its biological functions, signaling mechanisms, and potential therapeutic applications. Typically produced in mammalian or insect expression systems with tags (e.g., Fc, His, or GST) for purification and detection, the recombinant form retains ligand-binding activity and structural integrity. Researchers use it to investigate receptor-ligand interactions, Wnt pathway modulation, and crosstalk with other signaling networks like Notch and Hedgehog. Its role in diseases, particularly cancer, has drawn significant attention. Overexpression of FZD6 is linked to tumor progression, metastasis, and drug resistance in cancers such as squamous cell carcinoma and leukemia, making it a potential therapeutic target.
Additionally, FZD6 recombinant protein aids in developing biologics (e.g., antibodies) or small-molecule inhibitors to block aberrant Wnt signaling. Its applications extend to studying developmental disorders, tissue regeneration, and cell polarity defects. By enabling structural analysis (e.g., crystallography) and high-throughput screening, recombinant FZD6 accelerates both basic research and drug discovery efforts targeting Wnt-related pathologies.
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