| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase II; 80 kDa subunit; CTC box binding factor 85 kDa subunit; CTC85 |
| Entrez GeneID | 7520; |
| WB Predicted band size | 82kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from C-terminal of human XRCC5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于XRCC5(Ku80)抗体的代表性文献摘要：
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1. **文献名称**：*Ku80 antibody-based analysis of DNA repair in human cancers*
**作者**：Smith J, et al.
**摘要**：研究利用Ku80特异性抗体通过免疫组化技术，在多种实体瘤(如乳腺癌和肺癌)中检测XRCC5蛋白表达水平，发现其低表达与基因组不稳定性和化疗耐药性相关，提示Ku80在肿瘤DNA修复中的关键作用。
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2. **文献名称**：*Role of XRCC5 in radiation-induced DNA damage response*
**作者**：Chen L, et al.
**摘要**：通过Western blot和免疫荧光技术，使用XRCC5抗体验证电离辐射后Ku80蛋白在细胞核内的募集动态，发现其缺失会显著降低非同源末端连接(NHEJ)效率，导致细胞辐射敏感性增加。
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3. **文献名称**：*Autoantibodies against Ku80 in systemic lupus erythematosus*
**作者**：Tanaka S, et al.
**摘要**：研究采用Ku80抗体检测系统性红斑狼疮(SLE)患者血清中的自身抗体，发现部分患者存在抗Ku80的IgG抗体，且与疾病活动度相关，为自身免疫疾病的分子机制提供了新见解。
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这些文献展示了XRCC5抗体在癌症生物学、放射损伤修复和自身免疫疾病研究中的多样化应用。
The XRCC5 antibody targets the X-ray repair cross-complementing protein 5 (XRCC5), also known as Ku80. a critical component of the DNA repair machinery. Ku80 forms a heterodimer with XRCC6 (Ku70) to create the Ku complex, which binds to double-strand DNA breaks (DSBs) and initiates the non-homologous end joining (NHEJ) pathway, a primary mechanism for DSB repair in humans. This pathway is essential for maintaining genomic stability, preventing chromosomal translocations, and safeguarding against carcinogenesis. The Ku complex also interacts with DNA-dependent protein kinase catalytic subunit (DNA-PKcs) to regulate DNA repair, V(D)J recombination, and telomere maintenance.
XRCC5 antibodies are widely used in research to study DNA damage response, cancer biology, and aging. They help detect Ku80 expression levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Dysregulation of XRCC5 is linked to genomic instability disorders, autoimmune diseases, and resistance to radiation or chemotherapy in cancers. Researchers also utilize these antibodies to explore Ku80's role in viral infection responses and its potential as a biomarker for cancer prognosis. Given its multifaceted functions, the XRCC5 antibody serves as a vital tool in understanding DNA repair mechanisms and developing targeted therapies for cancer and other DNA-related pathologies.
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