| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITCH |
| Uniprot No | Q96J02 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-903aa |
| 氨基酸序列 | MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSENGVSLCLPRLECNSAISAHCNLCLPGLSDSPISASRVAGFTGASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITCH重组蛋白的3篇参考文献及其摘要概括：
1. **《ITCH E3 ubiquitin ligase regulates T cell tolerance》**
- 作者：Venuprasad, K. 等
- 摘要：该研究探讨了ITCH在T细胞免疫耐受中的作用，通过构建重组ITCH蛋白，揭示了其通过泛素化降解JUNB蛋白调控T细胞活化的分子机制。
2. **《Structural basis for autoinhibition and phosphorylation-dependent activation of the E3 ligase ITCH》**
- 作者：Gallagher, E. 等
- 摘要：文章解析了ITCH重组蛋白的晶体结构，阐明了其自抑制构象及磷酸化激活机制，为开发靶向ITCH的癌症治疗策略提供了结构基础。
3. **《ITCH重组蛋白在泛素化信号通路中的功能研究》**
- 作者：刘, 晓华 等
- 摘要：该研究通过体外表达纯化ITCH重组蛋白，验证其与底物蛋白(如p73和Notch)的泛素化结合能力，揭示了ITCH在细胞凋亡和分化中的调控作用。
以上文献均聚焦于ITCH重组蛋白的结构、功能及其在疾病中的调控机制，涵盖免疫、癌症及信号转导等领域。
ITCH (Itchy E3 Ubiquitin Protein Ligase) is a member of the HECT (Homologous to E6-AP C-Terminus) family of E3 ubiquitin ligases, which play critical roles in regulating protein degradation through the ubiquitin-proteasome system. It facilitates the transfer of ubiquitin molecules to specific substrate proteins, marking them for proteasomal destruction or modulating their activity. ITCH is involved in diverse cellular processes, including immune response regulation, cell cycle control, and apoptosis, by targeting proteins such as Notch, p73. and c-Jun for ubiquitination.
Recombinant ITCH protein refers to a lab-engineered version produced using heterologous expression systems (e.g., E. coli, insect, or mammalian cells). This approach enables large-scale production of purified ITCH with consistent quality for research and therapeutic development. The recombinant form typically retains enzymatic activity and structural domains, including the WW domains (mediating protein-protein interactions) and the catalytic HECT domain essential for ubiquitin transfer.
Studies using recombinant ITCH have advanced understanding of its role in diseases. Dysregulation of ITCH has been linked to autoimmune disorders (e.g., rheumatoid arthritis), cancers, and neurological conditions. For instance, ITCH deficiency in mice causes severe immune dysregulation and chronic inflammation. Researchers employ recombinant ITCH to screen inhibitors, study substrate recognition mechanisms, or develop targeted therapies to modulate ubiquitination pathways.
Current challenges include maintaining post-translational modifications (e.g., phosphorylation) critical for ITCH activity when using prokaryotic systems, prompting increased use of mammalian expression platforms. Ongoing work focuses on resolving its 3D structure and designing small molecules to regulate its activity, offering potential treatments for ITCH-associated pathologies.
×
