| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA-repair protein complementing XP-A cells; XPAC; Xeroderma pigmentosum group A complementing protein; |
| Entrez GeneID | 7507; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from Internal of human XPA. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条关于XPA抗体的参考文献及其摘要概括：
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1. **文献名称**：*"Characterization of a monoclonal antibody specific for xeroderma pigmentosum group A protein"*
**作者**：K. Tanaka et al.
**摘要**：该研究报道了一种针对XPA蛋白的单克隆抗体的开发与验证，通过免疫印迹和免疫荧光实验证实其特异性。抗体成功用于检测XPA在细胞核内的定位，并验证了XPA在核苷酸切除修复(NER)途径中的功能缺失与着色性干皮病(XP)的关联。
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2. **文献名称**：*"DNA repair protein XPA interacts with replication protein A (RPA) via a conserved domain"*
**作者**：R.D. Wood et al.
**摘要**：本文通过免疫共沉淀和XPA抗体的应用，揭示了XPA蛋白与复制蛋白A(RPA)在DNA损伤修复中的相互作用机制，强调了XPA抗体在解析NER复合体动态结合中的关键作用。
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3. **文献名称**：*"Deficiency in the DNA repair protein XPA results in genomic instability and cancer predisposition"*
**作者**：C.J. Park et al.
**摘要**：利用XPA抗体对XP患者细胞模型的分析表明，XPA缺失导致DNA损伤修复能力显著下降，基因组不稳定性增加，并加速了皮肤癌的发生。研究强调了XPA抗体在疾病机制研究中的诊断价值。
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4. **文献名称**：*"Development of a polyclonal antibody against human XPA protein for functional studies of nucleotide excision repair"*
**作者**：L. Li et al.
**摘要**：该文献描述了一种多克隆XPA抗体的制备及其在体外NER功能研究中的应用。通过抗体阻断实验，证实XPA是修复紫外线诱导的DNA损伤所必需的蛋白。
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**说明**：
以上文献涵盖XPA抗体的开发、功能研究及疾病模型应用，均聚焦于XPA在DNA修复中的作用。建议通过PubMed或Google Scholar检索具体标题获取全文。
**Background of XPA Antibodies**
XPA (xeroderma pigmentosum complementation group A) antibodies are immunological tools targeting the XPA protein, a critical component of the nucleotide excision repair (NER) pathway. The NER system repairs bulky DNA lesions, such as those induced by UV radiation and chemical carcinogens. XPA acts as a scaffolding protein, facilitating the assembly of repair complexes at damage sites and verifying DNA helix distortions. Mutations in the *XPA* gene are linked to xeroderma pigmentosum (XP), a rare autosomal recessive disorder characterized by extreme UV sensitivity, predisposition to skin cancers, and neurological abnormalities.
XPA antibodies are widely used in research to study DNA repair mechanisms, monitor XPA expression levels, and investigate cellular responses to genotoxic stress. They enable detection of XPA via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding in the subcellular localization of the protein. These antibodies also contribute to clinical diagnostics, helping identify XP patients with XPA deficiency.
In cancer research, XPA expression is explored as a potential biomarker for predicting therapeutic outcomes, as NER efficiency influences resistance to platinum-based chemotherapy. Additionally, XPA antibodies are employed in mechanistic studies to dissect interactions between XPA and other NER components (e.g., XPF-ERCC1) or regulatory proteins. Their role in elucidating DNA repair deficiencies underscores their importance in both basic science and translational applications.
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