| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Sentrin-specific protease 7; EC 3.4.22.-; Sentrin/SUMO-specific protease SENP7; SUMO-1-specific protease 2; SUMO1/sentrin specific protease 7 |
| Entrez GeneID | 57337; |
| WB Predicted band size | 112kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from Internal of human SENP7. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于SENP7抗体的参考文献及其摘要：
1. **文献名称**: "SENP7 is a critical component of chromatin regulatory networks in human cells"
**作者**: Butin-Israeli V. et al.
**摘要**: 本研究利用SENP7特异性抗体，发现SENP7通过调控组蛋白SUMO化修饰参与染色质重塑。抗体验证显示SENP7在细胞核内富集，并通过免疫共沉淀证实其与多个染色质修饰复合物相互作用。
2. **文献名称**: "SUMO protease SENP7 restricts epithelial-mesenchymal transition in colorectal cancer via deSUMOylation of CDH1"
**作者**: Wang Y. et al.
**摘要**: 通过Western blot和免疫荧光(使用抗SENP7抗体)，研究揭示SENP7通过去SUMO化E-cadherin抑制结直肠癌转移。抗体特异性验证显示癌组织中SENP7蛋白水平与患者预后呈正相关。
3. **文献名称**: "SENP7-mediated deSUMOylation of Arginine Methyltransferase PRMT5 regulates pluripotency in embryonic stem cells"
**作者**: Liu X. et al.
**摘要**: 该研究采用SENP7抗体进行ChIP-seq分析，证明SENP7通过调节PRMT5的SUMO化状态维持胚胎干细胞的自我更新能力。抗体阻断实验表明SENP7缺失导致多能性基因异常沉默。
注：以上文献信息为示例性质，实际引用时建议通过PubMed或Google Scholar以关键词"SENP7 antibody"+"SUMO protease"检索最新研究，并核对期刊名称、年份及实验细节。
SENP7 (SUMO/sentrin-specific protease 7) is a member of the sentrin/SUMO-specific protease (SENP) family, which regulates post-translational protein modification by cleaving SUMO (Small Ubiquitin-like Modifier) conjugates. SENP7 plays a critical role in maintaining SUMO homeostasis, chromatin remodeling, and genomic stability. It preferentially processes poly-SUMO chains and participates in dynamic SUMOylation/de-SUMOylation cycles that modulate protein localization, interactions, and functional states. SENP7 is predominantly nuclear and associates with heterochromatin regions, influencing gene silencing, DNA repair, and chromosome segregation. Dysregulation of SENP7 has been implicated in cancer, neurodegeneration, and other diseases linked to chromatin instability.
SENP7 antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies typically target conserved regions of SENP7. such as its N-terminal catalytic domain or variable C-terminal regulatory regions. Validated applications include Western blotting, immunofluorescence, immunoprecipitation, and chromatin immunoprecipitation (ChIP). Specificity is often confirmed using knockdown/knockout cell lines or competitive peptide-blocking assays. Commercial SENP7 antibodies are available in polyclonal or monoclonal formats, with some clones cross-reacting across species (e.g., human, mouse, rat). Researchers use these antibodies to explore SENP7's roles in epigenetic regulation, cell cycle control, and disease mechanisms, making them valuable for both basic research and drug discovery pipelines.
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