| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 3.4.22.-; SUMO1/sentrin specific peptidase 5; |
| Entrez GeneID | 205564; |
| WB Predicted band size | 86kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from Internal of human SENP5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于SENP5抗体的参考文献示例(注：以下内容为模拟虚构，实际文献需通过学术数据库检索验证)：
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1. **文献名称**: *"SENP5-mediated deSUMOylation regulates mitochondrial dynamics in cancer cells"*
**作者**: Yadav, S. et al. (2018)
**摘要**: 研究揭示了SENP5通过调控线粒体分裂蛋白(如DRP1)的SUMO化状态影响线粒体形态和癌细胞存活。研究使用SENP5特异性抗体进行Western blot和免疫荧光实验，证明其在前列腺癌细胞中的高表达与化疗耐药相关。
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2. **文献名称**: *"SENP5 modulates DNA damage response via controlling topoisomerase I SUMOylation"*
**作者**: Zhang, L. et al. (2020)
**摘要**: 本文发现SENP5通过去SUMO化拓扑异构酶I(TOP1)调控DNA损伤修复。利用SENP5抗体进行免疫共沉淀(Co-IP)和染色质定位分析，证实其在电离辐射后招募至DNA断裂位点，维持基因组稳定性。
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3. **文献名称**: *"SENP5 deficiency disrupts ribosomal biogenesis and leads to neurodevelopmental defects"*
**作者**: Lee, J. & Kim, H. (2019)
**摘要**: 通过构建SENP5基因敲除小鼠模型，结合SENP5抗体检测脑组织中的蛋白表达，发现SENP5缺失导致核糖体RNA加工异常，引发神经元发育障碍和小头畸形表型。
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**提示**：实际文献需通过PubMed、Google Scholar等平台以关键词“SENP5 antibody”或“SENP5 function”检索，并筛选实验方法中明确使用该抗体的研究。
The SENP5 (Sentrin/SUMO-specific protease 5) antibody is a key tool for studying the function and regulation of SENP5. a member of the SUMO protease family. SENP5 is involved in the post-translational modification of proteins through the removal of Small Ubiquitin-like Modifier (SUMO) groups, a process critical for regulating protein localization, stability, and interactions. Unlike other SENP family members, SENP5 preferentially processes SUMO-2/3 precursors and catalyzes deSUMOylation, influencing pathways such as mitosis, stress responses, and nucleolar functions. Its role in maintaining genomic stability and modulating cellular stress has linked it to diseases like cancer and neurodegenerative disorders.
SENP5 antibodies are typically generated using immunogenic peptides or recombinant protein fragments, designed to target specific epitopes of human or model organism SENP5. These antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect endogenous SENP5 expression, subcellular localization (e.g., nucleolar or cytoplasmic distribution), and dynamic changes under stress or during the cell cycle. Validation often involves knockout cell lines or siRNA-mediated knockdown to confirm specificity. As SENP5's activity is tightly regulated, its antibody also aids in studying post-translational modifications or interactions with binding partners. Research utilizing SENP5 antibodies has advanced understanding of SUMOylation dynamics in cellular homeostasis and pathology, making it essential for exploring therapeutic targets in SUMO-related diseases.
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