Rabbit Polyclonal NCoR1 Antibody

The nuclear receptor corepressor 1 (NCoR1) antibody is a crucial tool for studying transcriptional repression mechanisms mediated by the NCoR1 protein. NCoR1 is a core component of multiprotein complexes that recruit histone deacetylases (HDACs), such as HDAC3. to chromatin, enabling gene silencing by modifying histone structures and compacting DNA. It plays a pivotal role in regulating nuclear receptor signaling (e.g., thyroid hormone, retinoic acid receptors) and other transcription factors involved in development, metabolism, and immune responses. NCoR1 antibodies are widely used in techniques like Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence to detect protein expression, localization, and DNA-binding interactions. These antibodies help elucidate NCoR1's role in diseases, including cancer (e.g., leukemia, breast cancer), metabolic disorders, and developmental defects. Validated NCoR1 antibodies typically target specific epitopes within its conserved domains, such as the receptor interaction domain or deacetylase-binding region. Researchers rely on knockout cell lines or siRNA-mediated knockdown to confirm antibody specificity. Cross-reactivity with the homologous protein NCoR2/SMRT should be ruled out. Studies using NCoR1 antibodies have revealed its involvement in pathways like Notch signaling, inflammation (NF-κB regulation), and circadian rhythm control, underscoring its broad functional significance.