| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CA7 |
| Uniprot No | P43166 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-264aa |
| 氨基酸序列 | MTGHHGWGYG QDDGPSHWHK LYPIAQGDRQ SPINIISSQA VYSPSLQPLE LSYEACMSLS ITNNGHSVQV DFNDSDDRTV VTGGPLEGPY RLKQFHFHWG KKHDVGSEHT VDGKSFPSEL HLVHWNAKKY STFGEAASAP DGLAVVGVFL ETGDEHPSMN RLTDALYMVR FKGTKAQFSC FNPKCLLPAS RHYWTYPGSL TTPPLSESVT WIVLREPICI SERQMGKFRS LLFTSEDDER IHMVNNFRPP QPLKGRVVKA SFRA |
| 预测分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CA7(碳酸酐酶VII)重组蛋白研究的模拟参考文献示例。由于无法访问实时数据库，建议通过PubMed或Google Scholar检索真实文献：
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1. **标题**：**"High-Yield Expression and Purification of Recombinant Human Carbonic Anhydrase VII in Escherichia coli"**
**作者**：Zhang L, et al.
**摘要**：本研究优化了人源CA7在大肠杆菌中的重组表达，通过亲和层析纯化获得高纯度蛋白，并验证了其酶动力学特性，为后续结构-功能研究奠定基础。
2. **标题**：**"Crystal Structure of Recombinant CA7 Reveals Insights into Isoform-Specific Inhibitor Design"**
**作者**：Müller S, et al.
**摘要**：解析了重组CA7的晶体结构，发现其活性口袋与其他碳酸酐酶亚型的差异，为开发选择性抑制剂治疗CA7相关癫痫提供了结构依据。
3. **标题**：**"Functional Characterization of Recombinant CA7 in Zebrafish Model of Neurological Disease"**
**作者**：Garcia-Ruiz E, et al.
**摘要**：利用重组CA7蛋白在斑马鱼模型中恢复CA7缺陷表型，证明其在调节神经元pH平衡中的关键作用，提示其作为治疗靶点的潜力。
4. **标题**：**"Recombinant CA7-Based ELISA for Autoantibody Detection in Autoimmune Encephalitis"**
**作者**：Tanaka K, et al.
**摘要**：开发了基于重组CA7的ELISA检测方法，用于识别自身免疫性脑炎患者血清中的CA7抗体，提升了诊断特异性。
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**注意**：以上为模拟文献，实际研究中请通过学术数据库查询真实文献(如搜索关键词：**"recombinant CA7 protein"、"Carbonic Anhydrase VII expression"**)。真实文献可能涉及重组表达系统优化、疾病机制探索或药物开发等领域。
Carbonic Anhydrase VII (CA7), a member of the carbonic anhydrase (CA) enzyme family, plays a critical role in pH regulation and ion homeostasis by catalyzing the reversible hydration of carbon dioxide to bicarbonate and protons. Expressed predominantly in the brain, salivary glands, and gastrointestinal tract, CA7 is distinguished by its cytosolic localization and high catalytic efficiency. Its involvement in neuronal excitability and acid-base balance has linked it to neurological disorders, including epilepsy and migraine, where dysregulated pH exacerbates pathological conditions.
Recombinant CA7 protein is produced using biotechnological platforms (e.g., E. coli or mammalian expression systems) to ensure proper folding and post-translational modifications. This engineered protein retains native enzymatic activity, enabling researchers to study its structure-function relationships, inhibitor interactions, and physiological roles without isolating it from native tissues. Purification techniques like affinity chromatography yield high-purity CA7. essential for biochemical assays and crystallography studies.
Research on recombinant CA7 has accelerated drug discovery, particularly for CA isoform-specific inhibitors. Targeting CA7 holds therapeutic potential for neuropathic pain and seizure disorders, as selective inhibition could modulate neuronal pH without systemic side effects. Additionally, its overexpression in certain cancers has spurred interest in CA7 as a biomarker or therapeutic target. By providing a reliable tool for mechanistic studies, recombinant CA7 bridges gaps between molecular biology and clinical applications, underscoring its significance in both basic research and translational medicine.
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