| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CA14 |
| Uniprot No | Q9ULX7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 16-290aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSADGGQHWTYEGPHGQDHWPASYPECGN NAQSPIDIQTDSVTFDPDLPALQPHGYDQPGTEPLDLHNNGHTVQLSLPS TLYLGGLPRKYVAAQLHLHWGQKGSPGGSEHQINSEATFAELHIVHYDSD SYDSLSEAAERPQGLAVLGILIEVGETKNIAYEHILSHLHEVRHKDQKTS VPPFNLRELLPKQLGQYFRYNGSLTTPPCYQSVLWTVFYRRSQISMEQLE KLQGTLFSTEEEPSKLLVQNYRALQPLNQRMVFASFIQAGSSYTTGEM |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CA14重组蛋白的参考文献示例(注：以下内容为模拟示例，实际文献需根据真实数据库检索确认)：
1. **标题**: "Recombinant expression and functional analysis of human carbonic anhydrase XIV in HEK293 cells"
**作者**: Müller A, et al.
**摘要**: 研究报道了在HEK293细胞中成功表达人源CA14重组蛋白，并验证其碳酸酐酶活性及跨膜结构域对酶功能的影响。
2. **标题**: "Crystallographic characterization of recombinant CA XIV and its interaction with sulfonamide inhibitors"
**作者**: Park S, et al.
**摘要**: 通过重组表达纯化获得CA14蛋白，解析其晶体结构，揭示其与磺胺类抑制剂的结合模式，为药物设计提供依据。
3. **标题**: "Role of CA XIV in retinal pH regulation: Insights from recombinant protein studies"
**作者**: Alvarez BV, et al.
**摘要**: 利用重组CA14蛋白探究其在视网膜细胞中的酸碱调节功能，发现其与视觉信号传导的相关性。
4. **标题**: "Development of a high-yield E. coli system for recombinant CA XIV production"
**作者**: Chen L, et al.
**摘要**: 优化大肠杆菌表达体系，实现CA14重组蛋白的高效可溶性表达，为大规模制备提供技术方案。
建议通过PubMed或Web of Science以“CA14 recombinant protein”“Carbonic Anhydrase XIV expression”为关键词检索最新文献以获取准确信息。
CA14. or carbonic anhydrase XIV, is a member of the carbonic anhydrase (CA) family, a group of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate and protons. This enzyme family plays critical roles in pH regulation, ion transport, and metabolic processes across various tissues. CA14 is a membrane-associated isoform anchored via a glycosylphosphatidylinositol (GPI) linkage, predominantly expressed in the brain, retina, and kidney. It contributes to extracellular acid-base balance, neuronal signaling, and retinal function, with studies suggesting its involvement in visual transduction and cerebrospinal fluid production.
Recombinant CA14 protein is engineered to enable functional and structural studies, overcoming challenges in isolating native forms due to low tissue abundance and complex purification requirements. Typically produced in heterologous expression systems (e.g., E. coli, mammalian cells), recombinant CA14 retains enzymatic activity when properly folded and post-translationally modified. Its production often involves affinity tags (e.g., His-tag) for purification, followed by tag removal and validation via SDS-PAGE, Western blot, or activity assays using substrates like p-nitrophenyl acetate.
Research on recombinant CA14 focuses on elucidating its physiological roles and pathological implications. Dysregulation of CA14 has been linked to retinal disorders, neurological diseases, and cancers, making it a potential therapeutic target or biomarker. For instance, altered CA14 expression in glioblastoma and age-related macular degeneration highlights its clinical relevance. Additionally, recombinant CA14 serves as a tool for inhibitor screening, aiding drug development for CA-associated conditions. Its study also advances understanding of isoform-specific enzyme kinetics and substrate preferences, distinguishing it from other CA family members. Overall, recombinant CA14 bridges gaps in molecular mechanisms and translational applications within this enzyme family.
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