| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACYP2 |
| Uniprot No | P14621 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-99aa |
| 氨基酸序列 | STAQSLKSV DYEVFGRVQG VCFRMYTEDE ARKIGVVGWV KNTSKGTVTG QVQGPEDKVN SMKSWLSKVG SPSSRIDRTN FSNEKTISKL EYSNFSIRY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ACYP2重组蛋白的模拟参考文献示例(注：因文献数据库访问限制，以下内容为虚构示例，实际引用请核实真实文献)：
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1. **标题**: *"Recombinant ACYP2 Protein Enhances Neuronal Differentiation via MAPK Signaling"*
**作者**: Zhang L, et al.
**摘要**: 研究通过在大肠杆菌中表达重组人源ACYP2蛋白，发现其可激活MAPK通路，促进小鼠神经干细胞向神经元分化，提示ACYP2在神经发育中的潜在作用。
2. **标题**: *"Structural and Functional Characterization of ACYP2 Recombinant Protein in Cancer Cell Metabolism"*
**作者**: Kim S, Patel R.
**摘要**: 通过X射线晶体学解析了ACYP2重组蛋白的三维结构，并证明其通过调节ATP代谢抑制乳腺癌细胞增殖，为靶向ACYP2的癌症治疗提供依据。
3. **标题**: *"ACYP2 Recombinant Protein Attenuates Oxidative Stress in Cardiomyocytes"*
**作者**: Wang Y, et al.
**摘要**: 利用昆虫细胞系统表达ACYP2重组蛋白，发现其通过清除活性氧(ROS)保护心肌细胞免受氧化损伤，可能成为心血管疾病的治疗候选分子。
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**提示**：实际研究中，建议通过PubMed或Google Scholar搜索关键词“ACYP2 recombinant protein”或“acylphosphatase 2 expression”获取真实文献。
ACYP2 (Acylphosphatase 2) is a member of the acylphosphatase enzyme family, which catalyzes the hydrolysis of acylphosphates to corresponding carboxylates and inorganic phosphate. This small cytosolic protein, encoded by the ACYP2 gene located on human chromosome 2q37.3. plays a role in cellular energy metabolism and ion transport regulation. It shares structural homology with other acylphosphatases but exhibits distinct tissue expression patterns, with higher levels observed in skeletal muscle, brain, and certain cancer cells.
Research suggests ACYP2 may interact with proteins involved in ATPase activity and calcium signaling pathways, potentially influencing cellular homeostasis. Its recombinant form is typically produced using bacterial (e.g., E. coli) or mammalian expression systems, followed by purification via affinity chromatography and refolding processes. The recombinant protein maintains enzymatic activity and structural stability, enabling functional studies.
Interest in ACYP2 has grown due to its potential implications in human diseases. Elevated ACYP2 expression has been associated with tumor progression in various cancers, possibly through mechanisms involving energy metabolism reprogramming. Neurodegenerative studies propose its involvement in amyloid-beta aggregation processes relevant to Alzheimer's disease. Additionally, population genetics research links ACYP2 polymorphisms to human adaptation traits, including altitude tolerance and circadian rhythm variations.
As a research tool, recombinant ACYP2 facilitates biochemical characterization, inhibitor screening, and antibody production. Current investigations focus on elucidating its precise physiological substrates, regulatory networks, and therapeutic potential. The protein's small size (∼11 kDa) and stability make it particularly suitable for structural biology studies using NMR and X-ray crystallography. However, its exact biological significance remains partially understood, driving ongoing research into its multifunctional roles beyond basic enzymatic activity.
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