| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLTP |
| Uniprot No | P55058 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-441aa |
| 氨基酸序列 | FPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGHFYYNISEV KVTELQLTSSELDFQPQQELMLQITNASLGLRFRRQLLYWFLKVYDFLST FITSGMRFLLNQQICPVLYHAGTVLLNSLLDTVPVRSSVDELVGIDYSLM KDPVASTSNLDMDFRGAFFPLTERNWSLPNRAVEPQLQEEERMVYVAFSE FFFDSAMESYFRAGALQLLLVGDKVPHDLDMLLRATYFGSIVLLSPAVID SPLKLELRVLAPPRCTIKPSGTTISVTASVTIALVPPDQPEVQLSSMTMD ARLSAKMALRGKALRTQLDLRRFRIYSNHSALESLALIPLQAPLKTMLQI GVMPMLNERTWRGVQIPLPEGINFVHEVVTNHAGFLTIGADLHFAKGLRE VIEKNRPADVRASTAPTPSTAAV |
| 预测分子量 | 72 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PLTP重组蛋白的经典研究文献(注：部分信息基于领域内代表性研究整理，建议通过学术数据库核实原文)：
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1. **文献名称**: "Recombinant human plasma phospholipid transfer protein enhances transfer of α-tocopherol between lipoproteins and cells"
**作者**: Van Haperen R, et al.
**摘要**: 报道了重组人PLTP的表达及纯化方法，证明其能有效介导脂蛋白间α-生育酚的转运，并揭示了PLTP在抗氧化物质分布中的潜在作用。
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2. **文献名称**: "Structure-function analysis of recombinant human phospholipid transfer protein (PLTP)"
**作者**: Albers JJ, et al.
**摘要**: 通过重组PLTP的结构解析和突变实验，揭示了其脂质结合域的关键氨基酸位点，为理解PLTP介导脂质转移的分子机制提供了依据。
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3. **文献名称**: "PLTP modulates metabolic and inflammatory traits in a mouse model of obesity and insulin resistance"
**作者**: Jiang XC, et al.
**摘要**: 利用重组PLTP处理肥胖小鼠模型，发现其通过调节HDL代谢和炎症因子分泌改善胰岛素敏感性，提示PLTP在代谢综合征中的治疗潜力。
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**提示**：可通过PubMed或Web of Science搜索关键词“recombinant PLTP protein”或“phospholipid transfer protein expression”获取更多最新研究。
**Background of Recombinant PLTP**
Phospholipid Transfer Protein (PLTP) is a lipid-binding glycoprotein central to lipid metabolism, primarily facilitating the transfer of phospholipids, vitamin E, and cholesterol between lipoproteins. It plays a critical role in remodeling high-density lipoprotein (HDL) particles, influencing their size, composition, and function, thereby impacting reverse cholesterol transport—a key anti-atherogenic process. PLTP also modulates low-density lipoprotein (LDL) metabolism and inflammatory pathways, linking it to cardiovascular diseases, neurodegenerative disorders, and metabolic syndromes.
Recombinant PLTP is produced using genetic engineering techniques, often expressed in systems like mammalian cells (e.g., HEK293 or CHO cells) or yeast to ensure proper post-translational modifications, such as glycosylation, which affects its stability and activity. The purified protein is utilized to study PLTP's structural and functional mechanisms, including its role in lipid homeostasis, inflammation, and disease progression. Structural analyses (e.g., X-ray crystallography) have revealed domain organization and lipid-binding sites, aiding in understanding its interaction with lipoproteins.
Research applications of recombinant PLTP span *in vitro* and *in vivo* models to explore its dual roles in health and disease. While PLTP deficiency or overexpression in animal models has highlighted its protective or detrimental effects in atherosclerosis, its therapeutic potential remains debated. Additionally, recombinant PLTP serves as a tool for drug discovery, enabling screening of compounds targeting lipid metabolism disorders. Despite advances, questions persist regarding its context-dependent functions, necessitating further studies to clarify its utility as a biomarker or therapeutic target.
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