| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ketohexokinase; Hepatic fructokinase; KHK |
| Entrez GeneID | 3795; |
| WB Predicted band size | 33kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.90~95 (T-T-G-S-V) derived from Human KHK Isoform A . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于KHK Isoform A抗体的模拟参考文献示例(建议通过PubMed或Google Scholar获取真实文献)：
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1. **"Development of a Monoclonal Antibody Specific to Ketohexokinase Isoform A and Its Application in Metabolic Studies"**
*Author: Tanaka H, et al.*
摘要：本研究开发了一种高特异性识别KHK Isoform A的单克隆抗体，验证了其在Western blot和免疫组化中的应用，并发现KHK-A在肾脏和肠道中的表达显著高于肝脏，提示其可能参与果糖代谢的肠-肾轴调控。
2. **"Differential Roles of KHK Isoforms in Fructose-Induced Metabolic Syndrome"**
*Author: Johnson RL, et al.*
摘要：通过使用KHK Isoform A特异性抗体，作者发现KHK-A缺失可缓解高果糖饮食诱导的小鼠胰岛素抵抗，而KHK-C则主要驱动肝脏脂肪堆积，表明两种异构体在代谢综合征中的功能差异。
3. **"KHK-A Antibody-Based Detection Reveals Elevated Expression in Colorectal Cancer Tissues"**
*Author: Chen X, et al.*
摘要：利用新型KHK Isoform A抗体进行肿瘤组织分析，发现结直肠癌中KHK-A表达上调，且与患者预后不良相关，提示其可能作为癌症治疗的潜在靶点。
4. **"Characterization of KHK Isoform-Specific Antibodies and Their Implications in Neurological Disorders"**
*Author: Müller S, et al.*
摘要：研究对比了多种KHK异构体抗体的特异性，发现部分商用抗体存在交叉反应，而新开发的KHK-A抗体能精准识别脑组织中的目标蛋白，为神经退行性疾病中果糖代谢异常的研究提供了工具。
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注：以上为示例性内容，实际文献需通过学术数据库检索确认。
The KHK (ketohexokinase) Isoform A antibody is a specialized tool used to detect and study the A isoform of the ketohexokinase enzyme, which plays a critical role in fructose metabolism. KHK exists in two major splice variants, Isoform A (KHK-A) and Isoform C (KHK-C), differing in their tissue distribution and functional roles. While KHK-C is the primary isoform responsible for catalyzing fructose phosphorylation in the liver, kidneys, and intestines, KHK-A is expressed in tissues such as skeletal muscle, heart, and brain, though its enzymatic activity is significantly lower due to a 32-amino-acid deletion in its catalytic domain.
Research suggests that KHK-A may act as a regulatory molecule, potentially influencing metabolic pathways, cellular signaling, or interactions with other proteins, rather than directly participating in fructose metabolism. The KHK Isoform A antibody is essential for distinguishing between the two isoforms in experimental settings, enabling studies on their tissue-specific expression patterns and physiological roles. This antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate KHK-A's involvement in metabolic disorders, such as insulin resistance, non-alcoholic fatty liver disease (NAFLD), and diabetes, where aberrant fructose metabolism is implicated. By targeting unique epitopes in KHK-A, the antibody helps clarify its contribution to disease mechanisms and metabolic homeostasis.
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