| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CCR2; CKR2; CCR-2; CCR2A; CCR2B; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2 |
| Entrez GeneID | 729230 |
| clone | 3B6B1 |
| WB Predicted band size | 41.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD192 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于Acetyl-CoA Carboxylase (ACC1. Ab-79) 抗体的3篇参考文献示例(注：实际文献需根据抗体说明书或数据库核实，以下为模拟内容)：
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1. **文献名称**：*AMPK regulates energy expenditure by modulating acetyl-CoA carboxylase activity*
**作者**：Hardie, D.G. et al.
**摘要**：研究通过使用ACC1 (Ab-79) 抗体，证明了AMPK通过磷酸化ACC1的Ser79位点抑制其酶活性，从而减少脂肪酸合成并促进脂肪酸氧化，揭示了能量代谢调控的关键机制。
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2. **文献名称**：*Acetyl-CoA carboxylase phosphorylation predicts poor prognosis in breast cancer*
**作者**：Chajès, V. et al.
**摘要**：利用ACC1 (Ab-79) 抗体的免疫组化分析显示，肿瘤组织中ACC1的Ser79磷酸化水平与乳腺癌患者生存率负相关，提示ACC活性异常可能与癌症进展相关。
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3. **文献名称**：*High-fat diet induces ACC1 dephosphorylation via mTORC1 signaling*
**作者**：Brownsey, R.W. et al.
**摘要**：通过Western blot(使用Ab-79抗体)发现，高脂饮食通过mTORC1通路降低ACC1的Ser79磷酸化水平，导致肝脏脂质蓄积增加，为代谢综合征提供了分子机制解释。
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**备注**：
- 若需具体文献，建议参考抗体供应商(如CST、Abcam)官网提供的引用文献列表，或通过PubMed以“Acetyl-CoA Carboxylase phosphorylation Ser79”为关键词检索。
- Ab-79通常指针对ACC1蛋白第79位丝氨酸磷酸化位点的抗体。
Acetyl-CoA carboxylase (ACC) is a crucial enzyme in lipid metabolism, catalyzing the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. It exists in two isoforms: ACC1 (cytosolic) and ACC2 (mitochondrial-associated). ACC1 generates malonyl-CoA for de novo fatty acid synthesis, while ACC2-produced malonyl-CoA inhibits carnitine palmitoyltransferase-1 (CPT1), regulating mitochondrial fatty acid oxidation. Dysregulation of ACC activity is linked to metabolic disorders, obesity, and cancer, making it a therapeutic target.
The Acetyl-CoA Carboxylase (Ab-79) antibody is a monoclonal antibody specifically recognizing ACC, often targeting a conserved epitope across species. It is widely used in research to detect ACC expression levels, post-translational modifications (e.g., phosphorylation at Ser79), and subcellular localization via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Phosphorylation at Ser79 (detected by Ab-79) reflects ACC inactivation by AMP-activated protein kinase (AMPK), a key energy-sensing pathway, or protein kinase A (PKA), connecting cellular energy status to lipid metabolism.
This antibody is particularly valuable in studies exploring metabolic regulation, insulin resistance, and oncogenic pathways, as ACC overexpression or hyperactivity is observed in various cancers. Its specificity helps differentiate ACC isoforms or assess isoform-specific roles in tissue metabolism. Researchers also utilize Ab-79 to evaluate pharmacological ACC inhibitors in preclinical models, aiding drug development for metabolic syndrome and cancer therapeutics.
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