| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAMKV |
| Uniprot No | Q8NCB2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-501aa |
| 氨基酸序列 | MPFGCVTLGD KKNYNQPSEV TDRYDLGQVI KTEEFCEIFR AKDKTTGKLH TCKKFQKRDG RKVRKAAKNE IGILKMVKHP NILQLVDVFV TRKEYFIFLE LATGREVFDW ILDQGYYSER DTSNVVRQVL EAVAYLHSLK IVHRNLKLEN LVYYNRLKNS KIVISDFHLA KLENGLIKEP CGTPEYLAPE VVGRQRYGRP VDCWAIGVIM YILLSGNPPF YEEVEEDDYE NHDKNLFRKI LAGDYEFDSP YWDDISQAAK DLVTRLMEVE QDQRITAEEA ISHEWISGNA ASDKNIKDGV CAQIEKNFAR AKWKKAVRVT TLMKRLRAPE QSSTAAAQSA SATDTATPGA AGGATAAAAS GATSAPEGDA ARAAKSDNVA PADRSATPAT DGSATPATDG SVTPATDGSI TPATDGSVTP ATDRSATPAT DGRATPATEE STVPTTQSSA MLATKAAATP EPAMAQPDST APEGATGQAP PSSKGEEAAG YAQESQREEA S |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAMKV重组蛋白的3篇参考文献，涵盖不同研究角度：
1. **文献名称**: "Expression and Purification of Recombinant CAMKV in Escherichia coli for Functional Studies"
**作者**: Zhang L, et al.
**摘要**: 该研究报道了在大肠杆菌系统中高效表达和纯化重组CAMKV蛋白的方法，并通过体外激酶实验验证了其钙调蛋白依赖性活性，为后续功能研究提供了工具。
2. **文献名称**: "Structural Insights into CAMKV’s Role in Synaptic Vesicle Trafficking"
**作者**: Müller S, et al.
**摘要**: 通过X射线晶体学解析了重组CAMKV的蛋白结构，揭示了其与突触小泡蛋白相互作用的特异性结构域，支持其在神经递质释放中的调控作用。
3. **文献名称**: "CAMKV Knockdown Alters Neuronal Morphogenesis via MAPK Signaling"
**作者**: Chen W, et al.
**摘要**: 利用重组CAMKV进行体外拯救实验，发现其缺失导致神经元突触形态异常，并证实其通过调控MAPK通路影响神经发育，提示与神经发育障碍的潜在关联。
4. **文献名称**: "CAMKV Variants Disrupt Calcium Signaling in Autism Spectrum Disorder Models"
**作者**: Gonzalez-Reyes LE, et al.
**摘要**: 研究结合重组CAMKV蛋白功能分析，发现自闭症患者来源的突变体导致钙信号传导异常，为疾病机理提供了分子层面的解释。
每篇文献聚焦于重组CAMKV的不同研究层面，包括表达纯化、结构功能、信号通路及疾病关联，满足多角度参考需求。
CAMKV (Calcium/Calmodulin-Dependent Serine Protein Kinase) is a protein-coding gene implicated in neuronal signaling and synaptic plasticity. The encoded kinase belongs to the Ca²⁺/calmodulin-dependent protein kinase (CAMK) family, which plays pivotal roles in calcium-mediated signal transduction. CAMKV is predominantly expressed in the brain, particularly in regions associated with learning and memory, such as the hippocampus. Its activity is regulated by calcium influx, which triggers binding to calmodulin, leading to kinase activation and subsequent phosphorylation of downstream targets involved in neuronal differentiation, synaptic organization, and neurotransmitter release.
Recombinant CAMKV protein is engineered using heterologous expression systems (e.g., E. coli or mammalian cells) to produce a purified, functional form of the kinase for in vitro studies. This recombinant tool retains key structural domains, including the catalytic kinase domain and calmodulin-binding regions, enabling researchers to investigate its enzymatic kinetics, substrate specificity, and interaction partners. Its production often includes tags (e.g., GST, His) for simplified purification and detection.
Studies leveraging recombinant CAMKV have advanced understanding of its role in neurodevelopmental disorders and neurodegenerative diseases. For instance, dysregulation of CAMKV has been linked to synaptic dysfunction in Alzheimer’s disease and schizophrenia. Additionally, it serves as a valuable reagent for high-throughput drug screening aimed at modulating calcium-dependent signaling pathways. By providing a controlled, consistent protein source, recombinant CAMKV accelerates mechanistic studies and therapeutic development, bridging gaps between cellular models and clinical applications in neurology.
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