| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 5-HT2B, 5-HT(2B) |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human HTR2B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及p38 MAPK (Phospho-Thr180/Tyr182)抗体应用的关键参考文献，信息简明概括：
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1. **文献名称**：*SB 203580 is a specific inhibitor of a MAP kinase homologue which is stimulated by cellular stresses and interleukin-1*
**作者**：Cuenda A, et al. (1995)
**摘要**：该研究首次克隆并鉴定了p38 MAPK的激活机制，使用针对Thr180/Tyr182磷酸化位点的抗体(Ab-180/182)检测了氧化应激和炎症因子(如IL-1)诱导的p38 MAPK活化，并验证了SB203580抑制剂的特异性。
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2. **文献名称**：*Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine*
**作者**：Raingeaud J, et al. (1995)
**摘要**：研究通过Western blotting(使用Phospho-p38 Thr180/Tyr182抗体)证明p38 MAPK的激活需要T180/Y182双磷酸化，并揭示其在炎症因子(TNF-α、IL-1)及紫外线照射下的信号传导作用。
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3. **文献名称**：*A protein kinase involved in the regulation of inflammatory cytokine biosynthesis*
**作者**：Lee JC, et al. (1994)
**摘要**：该研究报道了p38 MAPK(当时称为CSBP)在LPS诱导巨噬细胞炎症反应中的关键作用，利用磷酸化特异性抗体(Thr180/Tyr182)证实其活化与TNF-α、IL-1β等细胞因子的产生直接相关。
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**备注**：上述文献为p38 MAPK研究的经典论文，均涉及该抗体的关键应用。如需近年研究或特定实验模型(如疾病研究)，可补充具体方向筛选文献。
The p38 mitogen-activated protein kinase (MAPK) is a serine/threonine kinase that plays a central role in cellular responses to stress, inflammation, apoptosis, and differentiation. Activation of p38 MAPK occurs through dual phosphorylation at conserved threonine (Thr180) and tyrosine (Tyr182) residues within the activation loop. The p38 MAPK (Ab-180/182) antibody is specifically designed to detect the phosphorylated form of p38 MAPK at these sites, serving as a critical tool for studying its activation status in various biological contexts.
This antibody is widely used in research to investigate signaling pathways involved in immune responses, cancer progression, neurodegeneration, and cardiovascular diseases. Its specificity for the phosphorylated Thr180/Tyr182 epitope allows researchers to distinguish activated p38 MAPK from its inactive form, enabling precise analysis of pathway dynamics under stimuli like cytokines, oxidative stress, or osmotic shock. Validation often includes knockout controls or pharmacological inhibitors to confirm target specificity. Applications span Western blotting, immunohistochemistry, flow cytometry, and immunofluorescence. The p38 MAPK (Ab-180/182) antibody has become indispensable in both basic research and drug discovery, particularly in studies targeting inflammatory disorders or evaluating therapeutic compounds modulating MAPK pathways.
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