| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | interferon regulatory factor 3, |
| Entrez GeneID | 3661; |
| WB Predicted band size | 45-55kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.394-398(H-I-S-N-S) derived from Human IRF-3. |
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以下是3篇涉及IRF-3(可能涵盖Ab-396抗体应用)的参考文献概览：
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1. **文献名称**：*Viral Infection Triggers the Phosphorylation of IRF-3 through TBK1*
**作者**：Sato M. et al.
**摘要**：研究揭示了病毒感染通过TBK1激酶介导IRF-3磷酸化的分子机制，利用IRF-3抗体(Ab-396)进行Western blot分析，证实磷酸化IRF-3在干扰素信号激活中的关键作用。
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2. **文献名称**：*IRF-3 Mediates Innate Immune Response against RNA Viruses*
**作者**：Lin R. et al.
**摘要**：通过免疫沉淀和染色质分析(使用IRF-3特异性抗体Ab-396)，证明IRF-3在识别RNA病毒后易位至细胞核，直接激活I型干扰素基因转录，揭示其抗病毒先天免疫中的核心地位。
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3. **文献名称**：*Regulation of IRF-3 Activation by Post-Translational Modifications*
**作者**：Hiscott J. et al.
**摘要**：探讨IRF-3的泛素化与磷酸化修饰对其活性的调控，采用Ab-396抗体进行免疫荧光实验，显示IRF-3的核转位动态及其与转录共激活因子的相互作用机制。
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**注**：若需具体文献来源或补充信息，建议通过PubMed或抗体供应商(如CST、Abcam)的产品引用列表进一步检索，结合抗体编号(如Ab-396)缩小范围。
IRF-3 (Interferon Regulatory Factor 3) is a critical transcription factor in the innate immune response, playing a central role in antiviral defense. It is activated upon detection of viral pathogens through pattern recognition receptors (PRRs), such as Toll-like receptors (TLRs) or RIG-I-like receptors (RLRs). Upon activation, IRF-3 undergoes phosphorylation, dimerization, and nuclear translocation, where it induces the expression of type I interferons (IFNs) and pro-inflammatory cytokines, initiating an antiviral state in infected and neighboring cells.
The IRF-3 (Ab-396) antibody is a specific monoclonal or polyclonal tool designed to detect endogenous levels of IRF-3 protein in various experimental applications, including Western blotting, immunofluorescence, and immunoprecipitation. Clone Ab-396 is often cited for its ability to recognize phosphorylated forms of IRF-3 (e.g., at Ser386 or Ser385. depending on species), a key marker of its activation during immune signaling. Researchers use this antibody to study IRF-3's regulatory mechanisms in viral infections, autoimmune diseases, and cancer, where dysregulated interferon responses are implicated.
Validation of IRF-3 (Ab-396) typically includes knockout cell line controls or siRNA-mediated knockdown to confirm specificity. Its utility in both basic and translational research underscores its importance in elucidating IRF-3-driven pathways and therapeutic targeting of interferon-related disorders.
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