| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DERMO1; MGC117334; bHLHa39 |
| Entrez GeneID | 117581; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.26~30(P-K-R-F-G) derived from Human Twist2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与Twist2抗体应用相关的文献摘要概括：
1. **文献名称**：*Twist2 contributes to breast cancer progression by promoting an epithelial-mesenchymal transition*
**作者**：Yang J. et al.
**摘要**：研究通过Western blot和免疫组化使用Twist2抗体，发现Twist2在乳腺癌中高表达，并通过调控EMT促进肿瘤转移。
2. **文献名称**：*The role of Twist2 in gastric cancer invasion and metastasis*
**作者**：Li H. et al.
**摘要**：利用Twist2抗体进行免疫荧光染色，证实Twist2通过激活Wnt/β-catenin通路增强胃癌细胞侵袭能力，与患者预后不良相关。
3. **文献名称**：*Twist2 is a novel prognostic marker in hepatocellular carcinoma*
**作者**：Wang Y. et al.
**摘要**：通过组织芯片结合Twist2抗体进行免疫组化分析，发现Twist2过表达与肝癌患者生存期缩短显著相关，提示其作为治疗靶点的潜力。
4. **文献名称**：*Twist2-mediated chemoresistance in ovarian cancer*
**作者**：Chen X. et al.
**摘要**：研究采用siRNA敲低和Twist2抗体检测，揭示Twist2通过抑制凋亡通路导致卵巢癌细胞对化疗药物耐药，为联合治疗提供依据。
以上研究均基于Twist2抗体在蛋白水平的功能验证，涵盖肿瘤转移、预后评估及耐药机制等多个方向。
Twist2. a member of the Twist family of basic helix-loop-helix (bHLH) transcription factors, plays critical roles in embryonic development and disease pathogenesis. It shares structural homology with Twist1 but exhibits distinct regulatory functions. During embryogenesis, Twist2 is essential for mesenchymal differentiation, neural crest cell migration, and skeletal development. In pathological contexts, Twist2 is implicated in epithelial-mesenchymal transition (EMT), a process driving cancer metastasis, fibrosis, and tissue remodeling. Overexpression of Twist2 in cancers (e.g., breast, gastric, and gliomas) correlates with poor prognosis, enhanced invasiveness, and chemoresistance. It suppresses epithelial markers (e.g., E-cadherin) and activates pro-metastatic pathways.
Twist2 antibodies are vital tools for studying its expression, localization, and function. These antibodies are used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to quantify Twist2 levels in tissues or cells, assess its nuclear/cytoplasmic distribution, and identify DNA-binding targets. Commercial Twist2 antibodies are typically raised against specific epitopes (e.g., human Twist2 N-terminal or C-terminal regions) in hosts like rabbits or mice. Validation includes testing reactivity in knockout models or siRNA-treated cells to confirm specificity. Researchers also employ Twist2 antibodies to explore its interplay with signaling pathways (e.g., TGF-β, Wnt) and potential as a therapeutic target. Reliable antibodies are crucial for elucidating Twist2's dual roles in development and disease.
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