| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E2F-2 |
| Entrez GeneID | 1870; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around aa.425~429(L-F-D-S-Y) derived from Human E2F-2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于E2F-2抗体的3篇参考文献及其摘要内容：
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1. **文献名称**：*"Identification of E2F-2-specific functional domains through the use of monoclonal antibodies"*
**作者**：Lees, J.A., Saito, M., & Harlow, E.
**摘要**：该研究通过制备针对E2F-2蛋白的单克隆抗体，鉴定了其功能结构域。抗体被用于免疫沉淀和Western blot分析，揭示了E2F-2在细胞周期调控中与其他转录因子(如DP-1)的相互作用，并确认其在G1/S期转换中的关键作用。
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2. **文献名称**：*"Regulation of E2F-2 by phosphorylation and pRB-binding during cell cycle progression"*
**作者**：Johnson, D.G., & Schneider-Broussard, R.
**摘要**：本研究利用E2F-2特异性抗体探究了其磷酸化状态及与视网膜母细胞瘤蛋白(pRB)的结合动态。结果显示，E2F-2在细胞周期不同阶段的功能受磷酸化修饰调控，且与pRB的相互作用影响其转录活性。
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3. **文献名称**：*"E2F-2 antibody-based chromatin immunoprecipitation reveals target genes in cancer cell proliferation"*
**作者**：Attwooll, C., Lazzerini Denchi, E., & Helin, K.
**摘要**：通过E2F-2抗体的染色质免疫共沉淀(ChIP)技术，鉴定了多种与癌症细胞增殖相关的E2F-2靶基因(如Cyclin E和CDK2)，并发现其在肿瘤发生中通过异常激活促癌通路。
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4. **文献名称**：*"Role of E2F-2 in hepatocellular carcinoma: Antibody-mediated knockdown and clinical implications"*
**作者**：Hernández-Muñoz, I., et al.
**摘要**：使用E2F-2抗体进行免疫组化分析，发现其在肝癌组织中高表达。通过抗体介导的蛋白敲低实验，证实E2F-2通过调控细胞周期相关基因促进肝癌细胞增殖和转移。
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这些文献涵盖了E2F-2抗体的应用场景(如功能研究、靶基因鉴定、临床相关性分析)，可为基础研究和疾病机制探索提供参考。
The E2F-2 antibody is a crucial tool for studying the E2F family of transcription factors, which regulate cell cycle progression, DNA repair, and apoptosis. E2F-2. a member of the activating E2F subgroup (E2F1-3), plays a pivotal role in driving the G1/S phase transition by promoting the expression of genes essential for DNA replication and cell cycle advancement, such as cyclin E and DNA polymerase. It functions in concert with the retinoblastoma protein (pRB), which modulates its activity through phosphorylation-dependent interactions. Dysregulation of E2F-2 is implicated in cancer, as overexpression can lead to uncontrolled proliferation, while mutations may disrupt normal cell cycle checkpoints.
The E2F-2 antibody is typically developed to specifically recognize and bind to the E2F-2 protein, enabling researchers to investigate its expression, localization, and interactions in various biological contexts. It is widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to explore E2F-2's role in transcriptional regulation, oncogenesis, and therapeutic responses. Commercial antibodies are often validated for specificity against recombinant E2F-2 or through knockout controls. Its application extends to cancer research, cell cycle studies, and drug development, offering insights into mechanisms underlying proliferation disorders and potential targets for anticancer therapies.
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