Rabbit Polyclonal Twist1 Antibody

Twist1 antibody is a crucial tool in biomedical research, primarily targeting the Twist1 protein, a basic helix-loop-helix (bHLH) transcription factor. Twist1 plays a pivotal role in embryonic development, particularly in mesoderm formation and neural tube closure. However, its dysregulation is strongly associated with cancer progression, metastasis, and epithelial-mesenchymal transition (EMT), a process enabling cancer cells to acquire invasive properties. Researchers use Twist1 antibodies to detect and quantify Twist1 expression in various experimental settings, including Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. These applications help elucidate Twist1's involvement in tumor microenvironment remodeling, drug resistance, and stem cell maintenance. Commercially available Twist1 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions). Validation across multiple species (human, mouse, rat) and sample types (tissue lysates, cell extracts) is critical for experimental reliability. Studies employing Twist1 antibodies have revealed its overexpression in aggressive cancers (e.g., breast, prostate, gastric), correlating with poor prognosis. However, interpretation requires caution due to potential cross-reactivity with other bHLH proteins and context-dependent post-translational modifications. Recent efforts focus on developing phospho-specific Twist1 antibodies to study its activated states in signaling pathways.