| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | c-RAF; RAF proto-oncogene serine/threonine-protein kinase; |
| Entrez GeneID | 24703; |
| WB Predicted band size | 74kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa. 641-645(T-S-P-R-L ) derived from Rat Raf-1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条关于Raf1抗体的参考文献及其摘要概括：
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1. **文献名称**：*Activation of the Raf/MAP kinase cascade by the Ras-related protein TC21 is required for the TC21-mediated transformation of NIH 3T3 cells*
**作者**：Marais, R. et al.
**摘要**：该研究利用Raf1特异性抗体，通过免疫沉淀和Western blot技术，揭示了TC21蛋白通过激活Raf1/MAPK信号通路促进细胞转化的分子机制，证实了Raf1在Ras依赖性肿瘤发生中的关键作用。
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2. **文献名称**：*The RAF family: an expanding network of post-translational controls and protein-protein interactions*
**作者**：Wellbrock, C. et al.
**摘要**：这篇综述系统总结了Raf家族蛋白(包括Raf1)的翻译后修饰调控及相互作用网络，重点讨论了Raf1抗体在检测其磷酸化状态(如Ser259和Ser338位点)及与MEK等下游效应分子结合研究中的应用。
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3. **文献名称**：*MAP kinase signalling pathways in cancer*
**作者**：Dhillon, A.S. et al.
**摘要**：文章分析了Raf1在癌症MAPK通路中的核心地位，通过Raf1抗体进行免疫组化染色，发现其在多种肿瘤组织中异常高表达，并验证了Raf1抑制剂与抗体联合使用在靶向治疗中的潜力。
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4. **文献名称**：*Raf-1-deficient mice exhibit increased ischemia-induced hippocampal neuron loss*
**作者**：Hüser, M. et al.
**摘要**：研究利用Raf1敲除小鼠模型，结合Raf1抗体进行脑组织免疫荧光分析，揭示了Raf1在神经元缺血损伤中的保护作用，为神经退行性疾病机制提供了新见解。
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以上文献均通过Raf1抗体在蛋白检测、定位或功能研究中发挥关键作用，涵盖肿瘤、信号通路及疾病模型等多个领域。
The Raf1 antibody is a crucial tool in molecular and cellular biology research, targeting the Raf1 protein (also known as C-Raf), a serine/threonine kinase within the MAPK/ERK signaling pathway. Raf1 plays a central role in regulating cell growth, differentiation, and survival by transmitting signals from cell surface receptors (e.g., Ras) to downstream effectors like MEK and ERK. Dysregulation of Raf1 is implicated in cancers, autoimmune disorders, and developmental defects, making it a key therapeutic target.
Raf1 antibodies are widely used to study protein expression, localization, and activation states in various biological contexts. They enable detection of Raf1 in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). Phospho-specific Raf1 antibodies detect phosphorylated forms (e.g., at Ser338 or Tyr340/341), providing insights into its activation during signaling events. Researchers also utilize these antibodies to investigate Raf1 interactions with binding partners like Ras, 14-3-3 proteins, or kinases in pathway crosstalk.
Commercial Raf1 antibodies are available as monoclonal or polyclonal variants, with validation often performed using knockout cell lines or siRNA-mediated depletion. Challenges include ensuring specificity due to structural similarities among Raf family members (A-Raf, B-Raf). Applications span basic research—such as dissecting oncogenic signaling in cancer models—and clinical studies exploring Raf1 as a biomarker or therapeutic target. Inhibitors targeting Raf1 (e.g., sorafenib) highlight its translational relevance, further driving demand for reliable Raf1 antibodies in drug development and mechanistic studies.
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