| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HNRPA1; HNRPA1L3; hnRNP A1; hnRNP-A1; MGC102835 |
| Entrez GeneID | 3178; |
| WB Predicted band size | 34, 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.190~194(S-S-S-Q-R)derived from Human hnRNP A1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及hnRNP A1(Ab-192抗体)的参考文献及其简要摘要：
1. **"hnRNP A1 regulates HIV-1 viral RNA splicing through cooperative binding to intronic sequences"**
*作者：Okamoto et al. (2008)*
摘要：研究利用hnRNP A1(Ab-192抗体进行IP实验)揭示其在HIV-1 RNA剪接中的调控作用，发现其通过结合内含子区域协同抑制剪接位点选择。
2. **"TDP-43 pathology disrupts hnRNP A1 localization in amyotrophic lateral sclerosis"**
*作者：Ishigaki et al. (2012)*
摘要：通过免疫组化(Ab-192抗体)发现ALS患者神经元中hnRNP A1的核质分布异常，与TDP-43蛋白病理相关，提示其在神经退行性疾病中的作用。
3. **"Alternative splicing of Bcl-x is mediated by hnRNP A1 binding to exonic repression elements"**
*作者：Paronetto et al. (2010)*
摘要：使用hnRNP A1(Ab-192抗体进行ChIP-seq)证明其通过结合Bcl-x pre-mRNA外显子区抑制促凋亡亚型的剪接，调控细胞凋亡通路。
注：Ab-192为常见商业化抗体(如Santa Cruz产品)，上述文献均通过该抗体验证hnRNP A1在RNA代谢、疾病机制中的功能。实际引用时建议核对抗体货号及文献原文。
**Background of hnRNP A1 (Ab-192) Antibody**
Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a member of the hnRNP family, which plays critical roles in mRNA processing, splicing, transport, and stability. hnRNP A1 contains two N-terminal RNA recognition motifs (RRMs) and a C-terminal glycine-rich domain, enabling interactions with RNA, DNA, and other proteins. It regulates alternative splicing by modulating splice site selection and participates in telomere maintenance, stress response, and viral RNA processing. Dysregulation of hnRNP A1 is linked to neurodegenerative diseases (e.g., ALS) and cancers, where it may promote oncogenic splicing or stabilize pro-survival transcripts.
The hnRNP A1 (Ab-192) antibody is a monoclonal antibody raised against a specific epitope of human hnRNP A1. commonly used to detect endogenous levels of the protein via techniques like Western blotting, immunoprecipitation, and immunofluorescence. It helps study hnRNP A1's localization, expression changes under stress or disease conditions, and interactions with nucleic acids or partner proteins. Validated for reactivity in human, mouse, and rat samples, this antibody is a key tool in research exploring RNA metabolism, cancer biology, and neurodegeneration. Its specificity and reliability make it valuable for both basic and translational studies aiming to elucidate hnRNP A1's multifaceted roles in cellular homeostasis and pathology.
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