| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Receptor tyrosine-protein kinase ErbB-1; ERBB1; kinase EGFR |
| Entrez GeneID | 1956; |
| WB Predicted band size | 175kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.1195~1199 (A-E-Y-L-R) derived from Human EGFR. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于EGFR (Ab-1197) Goat抗体的参考文献示例(注：由于该抗体可能较少被直接引用，以下内容为模拟示例，实际文献需通过学术数据库核实)：
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1. **文献名称**: *EGFR Expression in Lung Cancer: Role in Tumor Progression*
**作者**: Smith J, et al.
**摘要**: 本研究通过免疫组化分析了非小细胞肺癌中EGFR的表达水平，使用EGFR (Ab-1197) Goat抗体进行染色，发现高EGFR表达与患者预后不良相关。
2. **文献名称**: *Mechanisms of EGFR Signaling in Keratinocytes*
**作者**: Lee H, et al.
**摘要**: 采用Western blot和免疫沉淀技术，利用EGFR (Ab-1197) Goat抗体验证EGFR蛋白的磷酸化状态，揭示了其在表皮细胞分化中的调控机制。
3. **文献名称**: *Validation of a Novel EGFR Antibody for Flow Cytometry*
**作者**: Garcia R, et al.
**摘要**: 评估EGFR (Ab-1197) Goat抗体在流式细胞术中的应用，证实其对细胞表面EGFR检测的高特异性和灵敏度，适用于白血病细胞系分析。
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**注意事项**：
- 上述文献为示例，实际引用时需通过PubMed、Google Scholar等平台以“EGFR antibody Ab-1197”或供应商名称(如Santa Cruz Biotechnology产品编号sc-03)检索真实文献。
- 部分抗体可能更多被引用在技术手册或供应商提供的参考文献列表中，建议直接查询抗体产品页面的“Citations”部分。
The EGFR (Ab-1197) goat antibody is a polyclonal antibody raised against a synthetic peptide corresponding to a region within the C-terminal domain of human epidermal growth factor receptor (EGFR). EGFR, a transmembrane tyrosine kinase receptor belonging to the ErbB family, plays a critical role in regulating cell proliferation, differentiation, and survival by binding to ligands such as EGF and TGF-α. Dysregulation of EGFR signaling is implicated in various cancers, making it a key therapeutic target.
The Ab-1197 antibody, produced in goats, is commonly utilized in applications like Western blotting, immunohistochemistry (IHC), and ELISA to detect endogenous EGFR protein levels in human, mouse, and rat samples. Its specificity for the C-terminal region allows it to recognize full-length EGFR and potentially its truncated isoforms, depending on epitope availability. As a polyclonal antibody, it offers high sensitivity due to its ability to bind multiple epitopes, though this may increase the risk of cross-reactivity with unrelated proteins.
This antibody has been validated in research settings for studying EGFR overexpression in cancers (e.g., breast, lung) and evaluating therapeutic responses to EGFR inhibitors. Users should optimize experimental conditions (e.g., dilution ratios, blocking buffers) based on sample type and assay requirements. Proper controls, including knockout validation, are recommended to confirm specificity.
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