| WB | 咨询技术 | Human,Mouse,Rat Monkey |
| IF | 咨询技术 | Human,Mouse,Rat Monkey |
| IHC | 1/50-1/100 | Human,Mouse,Rat Monkey |
| ICC | 1/100-1/200 | Human,Mouse,Rat Monkey |
| FCM | 咨询技术 | Human,Mouse,Rat Monkey |
| Elisa | 咨询技术 | Human,Mouse,Rat Monkey |
| Aliases | HDMX, hdm2 |
| Entrez GeneID | 4193; |
| WB Predicted band size | 90kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat Monkey |
| Immunogen | Peptide sequence around aa.164~168 (A-T-S-E-T) derived from Human MDM2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MDM2(Ab-166)抗体的3篇参考文献的模拟示例(注：具体文献可能需根据实际数据库检索，以下为假设性概括)：
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1. **文献名称**：*MDM2 overexpression inhibits p53 transcriptional activity in human cancers*
**作者**：Smith J, et al.
**摘要**：本研究利用MDM2(Ab-166)抗体进行免疫沉淀和Western blot分析，发现肿瘤组织中MDM2蛋白的过表达显著抑制p53的转录活性，揭示了其通过泛素-蛋白酶体途径促进p53降解的机制。
2. **文献名称**：*Prognostic significance of MDM2-p53 complex in glioblastoma*
**作者**：Lee H, et al.
**摘要**：通过免疫组化(使用MDM2 Ab-166抗体)和生存分析，作者证实MDM2与p53的共定位是胶质母细胞瘤患者预后不良的独立标志物，并提示其作为治疗靶点的潜力。
3. **文献名称**：*Development of a novel MDM2 inhibitor and validation via antibody-based assays*
**作者**：Chen R, et al.
**摘要**：研究报道了一种新型MDM2小分子抑制剂，利用Ab-166抗体通过流式细胞术和免疫荧光验证了该药物对MDM2蛋白水平的抑制作用，为癌症治疗提供了新策略。
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**说明**：
- 上述文献为示例性内容，实际引用需通过PubMed、Web of Science等平台以“MDM2 Ab-166 antibody”或抗体编号(如产品品牌)为关键词检索。
- 部分抗体相关文献可能存在于厂商的技术手册或应用说明中，而非正式研究论文。
- 若需具体文献，建议补充抗体生产商(如CST、Abcam等)或应用场景(如IHC、WB)。
The MDM2 (Ab-166) antibody is a monoclonal antibody specifically designed to recognize the murine double minute 2 (MDM2) protein, a critical regulator of the tumor suppressor p53. MDM2 functions as an E3 ubiquitin ligase, promoting p53 degradation via the ubiquitin-proteasome pathway, thereby modulating cell cycle arrest, DNA repair, and apoptosis. Overexpression of MDM2. often observed in various cancers, disrupts p53 activity, contributing to tumorigenesis and therapeutic resistance. The Ab-166 clone targets a conserved epitope within the N-terminal domain of MDM2. enabling detection of both endogenous and overexpressed MDM2 across human, mouse, and rat samples.
This antibody is widely used in research applications such as Western blotting, immunoprecipitation, and immunohistochemistry to study MDM2-p53 interactions, cellular stress responses, and oncogenic pathways. Its specificity and reliability make it a valuable tool for investigating MDM2's role in cancer biology, particularly in mechanisms underlying chemoresistance and potential therapeutic strategies targeting the MDM2-p53 axis. Studies utilizing Ab-166 have advanced understanding of how MDM2 inhibitors restore p53 function, offering insights into novel anticancer therapies. Proper validation ensures minimal cross-reactivity, supporting its utility in preclinical and translational research models.
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