Rabbit Polyclonal c-Cbl(Ab-700) Antibody

The c-Cbl (Ab-700) antibody is a specific reagent targeting the phosphorylated tyrosine 700 (Tyr700) residue of the Casitas B-lineage lymphoma (c-Cbl) protein. c-Cbl, encoded by the *CBL* gene, is a multifunctional adaptor protein and E3 ubiquitin ligase involved in regulating signal transduction pathways, particularly receptor tyrosine kinase (RTK) downregulation. Upon activation via phosphorylation at Tyr700 (located in the linker region between the tyrosine kinase-binding domain and the proline-rich domain), c-Cbl recruits and ubiquitinates activated RTKs, marking them for lysosomal degradation. This process is critical for maintaining cellular homeostasis and preventing oncogenic signaling. Dysregulation of c-Cbl is implicated in cancers and immune disorders. The c-Cbl (Ab-700) antibody is widely used in immunoblotting (WB), immunoprecipitation (IP), and other applications to study c-Cbl activation, its role in ubiquitination-dependent pathways, and interactions with signaling molecules like EGFR, PDGFR, and ZAP-70. It is validated in detecting endogenous c-Cbl phosphorylated at Tyr700. often in contexts such as growth factor stimulation or immune cell activation. Researchers employ this antibody to explore mechanisms of oncogenesis, immune receptor modulation, and targeted protein degradation therapies. Its specificity is typically confirmed using cell lines (e.g., HEK293) treated with agonists inducing c-Cbl phosphorylation.