| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KIT; CD117; SCFR; PBT; |
| Entrez GeneID | 3815; |
| WB Predicted band size | 145kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.934~938 (H-I-Y-S-N) derived from Human c-Kit. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于c-kit (Ab-936)抗体的参考文献示例，内容基于典型应用场景和研究方向整理：
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1. **"c-KIT mutations in gastrointestinal stromal tumors: a critical review of the literature"**
*作者：Heinrich, M.C. et al.*
**摘要**：该研究分析了胃肠道间质瘤(GIST)中c-KIT基因的突变谱，并验证了c-kit抗体(如Ab-936)在免疫组化中的诊断价值，证实其高特异性与突变状态的相关性。
2. **"Immunohistochemical detection of c-KIT (CD117) in mast cell disorders: a comparative study of commercial antibodies"**
*作者：Horny, H.P. et al.*
**摘要**：比较多种c-kit抗体的性能，包括Ab-936.结果显示其在肥大细胞染色中灵敏度优异，适用于系统性肥大细胞增多症的病理诊断。
3. **"Role of c-KIT in hematopoietic stem cell maintenance and differentiation"**
*作者：Ding, L. & Morrison, S.J.*
**摘要**：探讨c-KIT信号在造血干细胞中的作用，使用Ab-936抗体通过流式细胞术和Western blot验证蛋白表达，支持其在干细胞自我更新中的关键功能。
4. **"Validation of anti-c-KIT antibodies for targeted therapy response assessment"**
*作者：Rubin, B.P. et al.*
**摘要**：评估c-kit抗体(包括Ab-936)在靶向药物治疗前后的表达变化，证明其作为治疗反应生物标志物的可靠性。
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**说明**：上述文献为示例性质，具体引用需根据实际研究来源调整。若需真实文献，建议检索PubMed或Web of Science，结合关键词“c-KIT Ab-936”或抗体编号(如供应商提供的引用文献列表)。
The c-kit (Ab-936) antibody is a monoclonal antibody specifically designed to detect the c-kit protein, a transmembrane receptor tyrosine kinase encoded by the KIT gene. This receptor plays a critical role in cellular signaling pathways regulating cell proliferation, survival, differentiation, and migration. It is activated by binding to its ligand, stem cell factor (SCF), leading to dimerization, autophosphorylation, and downstream activation of effectors like PI3K/AKT, MAPK, and JAK/STAT pathways. c-kit is highly expressed in hematopoietic stem cells, mast cells, melanocytes, and interstitial cells of Cajal, making it a key marker in studying these cell types.
The Ab-936 clone recognizes an epitope within the intracellular juxtamembrane domain of human c-kit, which is critical for receptor regulation and signaling. It is widely used in research applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate c-kit expression and activation in normal tissues and diseases. Dysregulation of c-kit, including mutations or overexpression, is implicated in cancers like gastrointestinal stromal tumors (GIST), melanoma, and acute myeloid leukemia (AML), making this antibody a valuable tool in oncology research. Additionally, it aids in studying stem cell biology and tissue regeneration mechanisms.
Validated for specificity and sensitivity, the Ab-936 antibody helps identify c-kit-positive cells in both diagnostic and experimental settings. Researchers often use it to explore therapeutic targets, as c-kit inhibitors are clinically relevant in treating certain malignancies. Proper controls and validation of phosphorylation status (if detecting activated c-kit) are recommended to ensure accurate interpretation.
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