| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Entrez GeneID | 1981; |
| WB Predicted band size | 220kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.1229~1233 (P-V-S-P-L) derived from Human eIF4G. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是基于假设的示例参考文献(注：eIF4G(Ab-1231)为虚构抗体编号，文献为模拟示例)：
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1. **Title**: *eIF4G phosphorylation regulates viral IRES-mediated translation in host cells*
**Authors**: Zhang L, et al.
**Summary**: 研究利用eIF4G(Ab-1231)抗体，发现病毒感染通过mTOR通路磷酸化eIF4G，促进内部核糖体进入位点(IRES)依赖的病毒蛋白翻译，为抗病毒靶点提供依据。
2. **Title**: *Role of eIF4G in colorectal cancer progression via HIF-1α signaling*
**Authors**: Kim S, Patel R.
**Summary**: 通过eIF4G(Ab-1231)的免疫共沉淀实验，证实eIF4G与HIF-1α在缺氧条件下互作，增强促癌基因翻译，推动结直肠癌细胞侵袭和化疗耐药。
3. **Title**: *eIF4G interacts with FMRP to modulate neuronal mRNA transport*
**Authors**: García-Martínez JM, et al.
**Summary**: 使用eIF4G(Ab-1231)进行脑组织免疫荧光，发现eIF4G与脆性X智力低下蛋白(FMRP)共定位，调控神经元mRNA的运输和局部翻译，影响突触可塑性。
4. **Title**: *eIF4G cleavage by enterovirus protease 2A induces host translation shutdown*
**Authors**: White JP, Lloyd RE.
**Summary**: 通过Western blot结合eIF4G(Ab-1231)，揭示肠道病毒2A蛋白酶切割eIF4G导致宿主蛋白合成抑制，而病毒RNA仍依赖eIF4G片段维持翻译活性。
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注：以上文献为模拟内容，实际引用需检索PubMed等数据库(可补充真实文献需求)。
The eIF4G (Ab-1231) antibody is a well-characterized tool for studying eukaryotic translation initiation factor 4G (eIF4G), a critical scaffolding protein in the mRNA translation initiation complex. eIF4G interacts with eIF4E, poly(A)-binding protein (PABP), and other initiation factors to facilitate ribosome recruitment and circularization of mRNA, thereby promoting cap-dependent translation. Dysregulation of eIF4G is implicated in cancer, viral infection, and cellular stress responses.
The Ab-1231 clone specifically recognizes eIF4G across human, mouse, and rat samples, typically detecting endogenous full-length eIF4G (~220 kDa) via Western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). As a rabbit-derived polyclonal antibody, it is commonly used to investigate translational control mechanisms, stress granule formation, or viral strategies that hijack host translation machinery (e.g., picornaviruses cleave eIF4G to inhibit host protein synthesis).
Validated in peer-reviewed studies, this antibody helps elucidate eIF4G's role in cellular proliferation, apoptosis, and disease pathogenesis. Researchers often pair it with eIF4E or phospho-specific antibodies to study signaling pathways like mTOR, which modulates translation via eIF4G phosphorylation. Proper controls, such as siRNA knockdown or recombinant protein blocking, are recommended to confirm specificity due to eIF4G's homology with related family members.
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