Rabbit Polyclonal eIF4B(Ab-422) Antibody

The eIF4B(Ab-422) antibody is a widely used tool in molecular biology to study the eukaryotic translation initiation factor 4B (eIF4B), a critical regulator of protein synthesis. eIF4B plays a key role in mRNA recruitment to ribosomes by enhancing the helicase activity of eIF4A, a component of the eIF4F complex, and promoting the scanning of mRNA for start codon recognition. It is phosphorylated at multiple sites, including Ser422. in response to growth signals, stress, or oncogenic activation, which modulates its activity and interaction with other translation machinery components. The eIF4B(Ab-422) antibody specifically recognizes eIF4B phosphorylated at Ser422. a modification linked to mTOR and MAPK signaling pathways. This phosphorylation event is associated with enhanced translation of mRNAs involved in cell proliferation, survival, and tumorigenesis. Researchers employ this antibody in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate eIF4B activation dynamics in cancer, metabolic diseases, and cellular stress responses. Its application helps elucidate mechanisms underlying translation dysregulation in pathological conditions and supports drug discovery targeting eIF4B-related signaling nodes. Validation typically includes testing in knockout models or phosphorylation-deficient mutants to ensure specificity.