| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRABP2 |
| Uniprot No | P29373 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-138aa |
| 氨基酸序列 | MPNFSGNWKI IRSENFEELL KVLGVNVMLR KIAVAAASKP AVEIKQEGDT FYIKTSTTVR TTEINFKVGE EFEEQTVDGR PCKSLVKWES ENKMVCEQKL LKGEGPKTSW TRELTNDGEL ILTMTADDVV CTRVYVRE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Crystal structure of recombinant human cellular retinoic acid-binding protein II (CRABP2) complexed with all-trans-retinoic acid"**
*Authors: Kleywegt GJ, Jones TA*
摘要：通过X射线晶体学解析了重组人CRABP2与全反式视黄酸结合的复合物结构，揭示了配体结合口袋的构象变化及关键氨基酸残基的相互作用机制。
2. **"CRABP2 enhances retinoic acid signaling by facilitating nuclear transport of retinoic acid"**
*Authors: Dong D, Noy N*
摘要：研究利用重组CRABP2蛋白验证其作为视黄酸转运载体的功能，证明其通过促进视黄酸向细胞核的转运，增强下游基因转录活性。
3. **"Recombinant CRABP2-FABP5 fusion protein as a potential drug delivery carrier for cancer therapy"**
*Authors: Budhu AS, Napoli JL*
摘要：构建CRABP2与FABP5的融合重组蛋白，验证其结合疏水性抗癌药物的能力，并展示其在体外增强药物靶向癌细胞的效果。
4. **"CRABP2 modulates MMP2 expression to promote cancer cell invasion via non-genomic retinoic acid signaling"**
*Authors: Sessler RJ, Noy N*
摘要：通过重组CRABP2蛋白实验，发现其通过非基因组信号通路调控基质金属蛋白酶MMP2的表达，进而促进癌细胞迁移和侵袭。
Cellular retinoic acid-binding protein 2 (CRABP2) is a small cytosolic protein belonging to the lipid-binding protein family, primarily involved in mediating the intracellular transport and metabolism of retinoic acid (RA), the active derivative of vitamin A. It facilitates the delivery of RA to nuclear retinoic acid receptors (RARs), enabling RA-regulated gene expression critical for cellular differentiation, proliferation, and embryonic development. Unlike its homolog CRABP1. CRABP2 is widely expressed in adult tissues and exhibits a stronger affinity for RA, positioning it as a key modulator of RA signaling pathways.
Recombinant CRABP2 protein is engineered through molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. Purification methods often involve affinity chromatography tags (e.g., His-tag) for high yield and purity. Structurally, the protein features a conserved β-barrel domain with a hydrophobic ligand-binding pocket, enabling selective RA binding and shielding it from non-specific enzymatic degradation.
Research on recombinant CRABP2 has illuminated its roles in cancer progression, skin homeostasis, and neurogenesis. Overexpression of CRABP2 has been linked to enhanced tumor invasiveness in certain cancers (e.g., breast, ovarian), while its interaction with RA pathways underscores therapeutic potential in dermatology (e.g., psoriasis, acne) and oncology (e.g., acute promyelocytic leukemia). Additionally, recombinant CRABP2 serves as a tool to study RA metabolism, receptor crosstalk, and ligand-mediated gene regulation, offering insights into drug design targeting RA-dependent diseases. Its biochemical stability and functional versatility make it a valuable reagent for both basic research and biopharmaceutical applications.
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