| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITGA6 |
| Uniprot No | P23229 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1130aa |
| 氨基酸序列 | MAAAGQLCLLYLSAGLLSRLGAAFNLDTREDNVIRKYGDPGSLFGFSLAMHWQLQPEDKRLLLVGAPRAEALPLQRANRTGGLYSCDITARGPCTRIEFDNDADPTSESKEDQWMGVTVQSQGPGGKVVTCAHRYEKRQHVNTKQESRDIFGRCYVLSQNLRIEDDMDGGDWSFCDGRLRGHEKFGSCQQGVAATFTKDFHYIVFGAPGTYNWKGIVRVEQKNNTFFDMNIFEDGPYEVGGETEHDESLVPVPANSYLGLLFLTSVSYTDPDQFVYKTRPPREQPDTFPDVMMNSYLGFSLDSGKGIVSKDEITFVSGAPRANHSGAVVLLKRDMKSAHLLPEHIFDGEGLASSFGYDVAVVDLNKDGWQDIVIGAPQYFDRDGEVGGAVYVYMNQQGRWNNVKPIRLNGTKDSMFGIAVKNIGDINQDGYPDIAVGAPYDDLGKVFIYHGSANGINTKPTQVLKGISPYFGYSIAGNMDLDRNSYPDVAVGSLSDSVTIFRSRPVINIQKTITVTPNRIDLRQKTACGAPSGICLQVKSCFEYTANPAGYNPSISIVGTLEAEKERRKSGLSSRVQFRNQGSEPKYTQELTLKRQKQKVCMEETLWLQDNIRDKLRPIPITASVEIQEPSSRRRVNSLPEVLPILNSDEPKTAHIDVHFLKEGCGDDNVCNSNLKLEYKFCTREGNQDKFSYLPIQKGVPELVLKDQKDIALEITVTNSPSNPRNPTKDGDDAHEAKLIATFPDTLTYSAYRELRAFPEKQLSCVANQNGSQADCELGNPFKRNSNVTFYLVLSTTEVTFDTPDLDINLKLETTSNQDNLAPITAKAKVVIELLLSVSGVAKPSQVYFGGTVVGEQAMKSEDEVGSLIEYEFRVINLGKPLTNLGTATLNIQWPKEISNGKWLLYLVKVESKGLEKVTCEPQKEINSLNLTESHNSRKKREITEKQIDDNRKFSLFAERKYQTLNCSVNVNCVNIRCPLRGLDSKASLILRSRLWNSTFLEEYSKLNYLDILMRAFIDVTAAAENIRLPNAGTQVRVTVFPSKTVAQYSGVPWWIILVAILAGILMLALLVFILWKCGFFKRSRYDDSVPRYHAVRIRKEEREIKDEKYIDNLEKKQWITKWNENESYS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITGA6重组蛋白的3篇参考文献示例(内容为虚构概括，仅供参考)：
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1. **文献名称**：*Expression and Purification of Recombinant ITGA6 in Mammalian Cells for Functional Studies*
**作者**：Zhang L, et al.
**摘要**：研究报道了在HEK293细胞中高效表达和纯化ITGA6重组蛋白的方法，通过亲和层析和尺寸排阻色谱获得高纯度蛋白。该蛋白在体外实验中证实可与层粘连蛋白(laminin)结合，并促进上皮细胞的粘附和迁移。
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2. **文献名称**：*Structural Insights into ITGA6-ITGB4 Heterodimerization Using Cryo-EM*
**作者**：Smith JR, et al.
**摘要**：利用冷冻电镜技术解析了ITGA6与ITGB4亚基形成的整合素异源二聚体结构，揭示了其与细胞外基质结合的分子机制，为靶向ITGA6的癌症治疗策略提供了结构基础。
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3. **文献名称**：*ITGA6 Recombinant Protein Enhances Stem Cell Expansion in 3D Culture Systems*
**作者**：Wang Y, et al.
**摘要**：研究表明，将重组ITGA6蛋白包被于水凝胶支架可显著提高人多能干细胞(hPSCs)的增殖和定向分化效率，提示其在组织工程和再生医学中的应用潜力。
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(注：以上文献为示例性内容，非真实存在的论文。)
Integrin alpha-6 (ITGA6), a member of the integrin family of cell adhesion receptors, plays a critical role in mediating interactions between cells and the extracellular matrix (ECM). It typically pairs with β subunits (e.g., ITGB1 or ITGB4) to form heterodimeric receptors, such as α6β1 and α6β4 integrins. These complexes bind laminins, key ECM components, facilitating cell adhesion, migration, and signaling. ITGA6 is widely expressed in epithelial, endothelial, and stem cells, contributing to tissue development, homeostasis, and repair. Notably, the α6β4 integrin is essential for hemidesmosome formation, anchoring epithelial cells to the basement membrane and maintaining tissue integrity.
Recombinant ITGA6 proteins are engineered to study its structure-function relationships and therapeutic potential. Produced via heterologous expression systems (e.g., mammalian or insect cells), these proteins often retain functional domains, such as the ligand-binding extracellular region, enabling in vitro analysis of integrin-ECM interactions. Researchers use recombinant ITGA6 to investigate its role in pathologies, including cancer metastasis, where overexpression correlates with tumor invasion and poor prognosis. It also serves as a tool to explore stem cell behavior, as ITGA6 is a marker of certain stem cell populations and regulates their self-renewal and differentiation.
In disease contexts, ITGA6 dysregulation is linked to epidermolysis bullosa, fibrosis, and aggressive cancers. Recombinant proteins aid in developing targeted therapies, such as blocking antibodies or peptides, to inhibit ITGA6-mediated signaling. Additionally, they support drug screening platforms and biomarker discovery. Beyond therapeutics, recombinant ITGA6 is utilized in tissue engineering to mimic ECM environments for regenerative applications. Its study continues to uncover mechanisms underlying cell-ECM communication, offering insights into both basic biology and translational medicine.
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