| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITGA8 |
| Uniprot No | P53708 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1012aa |
| 氨基酸序列 | MSPGASRGPR GSQAPLIAPL CCAAAALGML LWSPACQAFN LDVEKLTVYS GPKGSYFGYA VDFHIPDART ASVLVGAPKA NTSQPDIVEG GAVYYCPWPA EGSAQCRQIP FDTTNNRKIR VNGTKEPIEF KSNQWFGATV KAHKGKVVAC APLYHWRTLK PTPEKDPVGT CYVAIQNFSA YAEFSPCRNS NADPEGQGYC QAGFSLDFYK NGDLIVGGPG SFYWQGQVIT ASVADIIANY SFKDILRKLA GEKQTEVAPA SYDDSYLGYS VAAGEFTGDS QQELVAGIPR GAQNFGYVSI INSTDMTFIQ NFTGEQMASY FGYTVVVSDV NSDGLDDVLV GAPLFMEREF ESNPREVGQI YLYLQVSSLL FRDPQILTGT ETFGRFGSAM AHLGDLNQDG YNDIAIGVPF AGKDQRGKVL IYNGNKDGLN TKPSQVLQGV WASHAVPSGF GFTLRGDSDI DKNDYPDLIV GAFGTGKVAV YRARPVVTVD AQLLLHPMII NLENKTCQVP DSMTSAACFS LRVCASVTGQ SIANTIVLMA EVQLDSLKQK GAIKRTLFLD NHQAHRVFPL VIKRQKSHQC QDFIVYLRDE TEFRDKLSPI NISLNYSLDE STFKEGLEVK PILNYYRENI VSEQAHILVD CGEDNLCVPD LKLSARPDKH QVIIGDENHL MLIINARNEG EGAYEAELFV MIPEEADYVG IERNNKGFRP LSCEYKMENV TRMVVCDLGN PMVSGTNYSL GLRFAVPRLE KTNMSINFDL QIRSSNKDNP DSNFVSLQIN ITAVAQVEIR GVSHPPQIVL PIHNWEPEEE PHKEEEVGPL VEHIYELHNI GPSTISDTIL EVGWPFSARD EFLLYIFHIQ TLGPLQCQPN PNINPQDIKP AASPEDTPEL SAFLRNSTIP HLVRKRDVHV VEFHRQSPAK ILNCTNIECL QISCAVGRLE GGESAVLKVR SRLWAHTFLQ RKNDPYALAS LVSFEVKKMP YTDQPAKLPE GSIVIKTSVI WATPNVSFSI PL |
| 预测分子量 | 113 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITGA8重组蛋白的3篇代表性文献示例(注：内容为模拟概括，非真实文献)：
1. **文献名称**：*Structural and functional characterization of recombinant human integrin α8β1*
**作者**：Smith et al.
**摘要**：本研究通过哺乳动物表达系统成功制备了重组ITGA8/β1整合素蛋白，解析了其与纤连蛋白(fibronectin)结合的分子机制，并验证了其在细胞黏附和迁移中的关键作用。
2. **文献名称**：*ITGA8-mediated signaling regulates kidney development in a mouse model*
**作者**：Johnson et al.
**摘要**：利用重组ITGA8蛋白进行功能阻断实验，发现ITGA8通过与胞外基质蛋白相互作用调控肾小管形成，揭示了其在胚胎肾脏发育中的必要性。
3. **文献名称**：*Recombinant ITGA8 as a potential biomarker in cancer progression*
**作者**：Lee et al.
**摘要**：通过表达纯化的重组ITGA8蛋白，发现其在肿瘤微环境中促进血管生成，并可能作为上皮-间质转化(EMT)的分子标记物，为癌症治疗提供新靶点。
4. **文献名称**：*Optimization of ITGA8 extracellular domain expression in E. coli*
**作者**：Brown et al.
**摘要**：报道了一种高效的大肠杆菌表达系统用于生产ITGA8胞外段重组蛋白，并对其纯化条件及抗原性进行了系统分析，为后续抗体开发奠定基础。
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**提示**：实际研究中建议通过PubMed、Google Scholar等平台检索最新文献，关键词可包括“recombinant ITGA8”、“integrin alpha 8 expression”或“ITGA8 functional studies”。
**Background of ITGA8 Recombinant Protein**
Integrin alpha-8 (ITGA8) is a transmembrane glycoprotein belonging to the integrin family, which mediates cell-cell and cell-extracellular matrix (ECM) interactions. It typically pairs with the β1 subunit (ITGB1) to form the α8β1 heterodimer, a receptor for ECM ligands such as fibronectin, tenascin-C, and nephronectin. ITGA8 plays critical roles in organogenesis, tissue morphogenesis, and cellular adhesion, particularly during embryonic development. It is highly expressed in smooth muscle cells, kidney podocytes, and neural tissues, influencing processes like renal glomerulus formation, lung branching, and neuronal guidance.
Recombinant ITGA8 protein is engineered *in vitro* using expression systems (e.g., mammalian or insect cells) to produce soluble, functional forms of the protein, often excluding the transmembrane domain for ease of purification and application. This protein retains ligand-binding activity, enabling studies on integrin-mediated signaling, cell adhesion, and migration. Researchers utilize it to investigate ITGA8's involvement in diseases, including chronic kidney disease, fibrosis, and cancer metastasis. For instance, ITGA8 may act as a tumor suppressor or promoter depending on the cancer type, highlighting its context-dependent roles.
The recombinant protein is typically purified via affinity tags (e.g., His-tag) and validated for bioactivity through ligand-binding assays. Its applications span *in vitro* cell culture models, mechanistic studies of integrin-ECM interactions, and drug discovery platforms targeting integrin-related pathologies. By elucidating ITGA8's structure-function relationships and signaling pathways, this tool advances understanding of tissue homeostasis and disease mechanisms, offering potential therapeutic avenues for regenerative medicine and cancer treatment.
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