Rabbit Polyclonal p90RSK(Ab-348) Antibody

The p90RSK (Ribosomal S6 Kinase) family, encoded by the RPS6KA genes, comprises serine/threonine kinases activated downstream of the MAPK/ERK signaling pathway. These kinases play critical roles in regulating cell proliferation, survival, differentiation, and apoptosis. p90RSK contains two kinase domains (N-terminal and C-terminal) linked by a regulatory region, with activation requiring sequential phosphorylation by ERK1/2 and PDK1. The Ab-348 antibody specifically targets p90RSK, often recognizing a conserved epitope within its C-terminal kinase domain or phosphorylation sites (e.g., Ser380), depending on the immunogen design. As a monoclonal antibody, it is widely used in Western blotting, immunofluorescence, and immunoprecipitation to study p90RSK expression, activation, and subcellular localization. Its specificity makes it valuable for investigating MAPK/ERK signaling dynamics in diseases like cancer, where aberrant RSK activity is linked to tumor progression and therapy resistance. Researchers rely on Ab-348 to assess RSK phosphorylation states, interactions with downstream targets (e.g., CREB, Bad), and responses to pathway inhibitors. Validation typically includes knockout controls or peptide blocking to confirm minimal cross-reactivity with related kinases (e.g., RSK2/3). This antibody has been instrumental in elucidating RSK's dual role in pro-survival signaling and feedback regulation of upstream pathways.