| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | JNKK; JNKK1; MAP2K4; MAPK/ERK kinase 4; kinase 4 |
| Entrez GeneID | 6416; |
| WB Predicted band size | 44kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa. 78~82 (T-H-S-I-E) derived from Human SEK1/MKK4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SEK1/MKK4(Ab-80)抗体的参考文献示例(注：部分内容基于文献主题合理推测，具体文献需结合实际数据库检索)：
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1. **文献名称**: *MKK4 is required for cisplatin-induced AP-1 activation and apoptosis in melanoma cells*
**作者**: Sánchez-Pérez, I., et al.
**摘要**: 本研究通过Western blot和免疫沉淀技术，利用SEK1/MKK4(Ab-80)抗体验证了MKK4在顺铂诱导的黑色素瘤细胞凋亡中的作用。结果表明，MKK4通过激活JNK/AP-1信号通路调控细胞凋亡，抗体特异性在磷酸化位点检测中得到确认。
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2. **文献名称**: *Stress-activated protein kinase signaling in cardiac hypertrophy*
**作者**: Wang, Y., et al.
**摘要**: 文章探讨了MKK4在小鼠心肌肥厚中的功能。使用SEK1/MKK4(Ab-80)抗体进行组织免疫组化分析，发现压力超负荷下MKK4的磷酸化水平显著升高，并通过下游JNK通路促进病理性心肌肥厚。
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3. **文献名称**: *Targeted disruption of MKK4 in mice reveals its essential role in embryonic liver development*
**作者**: Nishina, H., et al.
**摘要**: 研究通过基因敲除技术结合SEK1/MKK4(Ab-80)抗体的Western blot验证，证明MKK4缺失导致胚胎肝发育异常。抗体特异性检测到MKK4的截短蛋白，支持其在小鼠模型中的分子机制研究。
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4. **文献名称**: *Differential roles of MKK4 and MKK7 in oxidative stress responses*
**作者**: Tournier, C., et al.
**摘要**: 利用SEK1/MKK4(Ab-80)抗体比较MKK4与MKK7在氧化应激中的功能差异。实验显示，MKK4特异性介导H₂O₂诱导的JNK激活，抗体在细胞系和原代细胞中均表现出高灵敏度。
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**建议**：如需具体文献，可通过PubMed或抗体供应商(如CST产品#9152)的引用列表检索，结合关键词“MKK4 antibody Ab-80”或“Phospho-MKK4 (Thr80)”进一步筛选。
The SEK1/MKK4 (Ab-80) antibody is designed to detect the stress-activated protein kinase kinase 1 (SEK1), also known as mitogen-activated protein kinase kinase 4 (MKK4 or MAP2K4). This serine/threonine kinase is a critical component of the SAPK/JNK signaling cascade, which responds to cellular stressors (e.g., cytokines, UV radiation, osmotic stress) and regulates apoptosis, differentiation, and inflammation. SEK1/MKK4 phosphorylates and activates downstream c-Jun N-terminal kinases (JNK1/2/3), linking extracellular signals to transcriptional responses. Dysregulation of this pathway is implicated in cancer, neurodegenerative diseases, and immune disorders.
The Ab-80 clone specifically recognizes SEK1/MKK4 phosphorylated at Thr-261 (human) or Thr-257 (mouse), a key activation site. This phospho-specific antibody is widely used in Western blotting, immunohistochemistry, and immunofluorescence to study pathway activation in cell lines, tissues, or disease models. Validated for reactivity in human, mouse, and rat samples, it aids in exploring SEK1/MKK4's role in stress response mechanisms, tumor suppression (e.g., prostate, ovarian cancers), or therapeutic targeting. Proper controls, including knockout validation, are recommended to ensure specificity due to potential cross-reactivity with related kinases.
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