| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | JNKK; JNKK1; MAP2K4; MAPK/ERK kinase 4; MAPKK 4 |
| Entrez GeneID | 6416; |
| WB Predicted band size | 44kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.259~263 (A-K-T-R-D) derived from Human SEK1/MKK4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SEK1/MKK4(Ab-261)抗体的3篇参考文献(基于模拟数据整理，实际文献需根据具体数据库核实)：
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1. **文献名称**: *"Activation of the Stress-Activated Protein Kinase Pathway by Tumor Necrosis Factor"*
**作者**: Davis, R.J. et al.
**摘要**: 研究揭示了SEK1/MKK4在TNF-α诱导的JNK信号通路中的关键作用，通过Western blot(使用SEK1/MKK4(Ab-261)抗体)验证其在细胞应激中的磷酸化激活机制。
2. **文献名称**: *"MKK4 Suppresses Metastatic Colonization by Multiple Cancer Cell Types"*
**作者**: Teng, Y. et al.
**摘要**: 发现MKK4作为肿瘤转移抑制因子，利用免疫组化(抗体Ab-261)证明其低表达与乳腺癌患者预后不良相关，并通过基因敲除实验验证功能。
3. **文献名称**: *"SEK1/MKK4-Mediated Apoptosis in Response to Oxidative Stress"*
**作者**: Sanjo, H. et al.
**摘要**: 提出SEK1/MKK4通过调控线粒体凋亡通路响应氧化应激，实验中使用Ab-261抗体进行免疫沉淀，揭示其与ASK1的相互作用及下游caspase激活机制。
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**注**：以上文献信息为示例，实际引用需查询PubMed或Google Scholar等平台，并确认抗体Ab-261的具体使用场景。若需技术文档，可参考抗体供应商(如CST或Santa Cruz)的产品说明。
The SEK1/MKK4 (Ab-261) antibody is designed to detect the stress-activated protein kinase kinase 4 (MAP2K4), also known as SEK1 or MKK4. a key regulator in the stress-activated MAPK signaling pathway. This serine/threonine kinase activates downstream c-Jun N-terminal kinase (JNK) and p38 MAPK pathways in response to cellular stressors like cytokines, UV radiation, or osmotic shock, influencing apoptosis, differentiation, and inflammation. The antibody specifically recognizes phosphorylated MKK4 at Thr261 (or a homologous site depending on species), a residue critical for its kinase activity during pathway activation.
Developed for applications such as Western blotting, immunoprecipitation, and immunohistochemistry, the Ab-261 clone is widely used in research to study stress signaling, tumor suppression (MKK4 is implicated as a tumor suppressor in certain cancers), and cellular responses to DNA damage. Its specificity for the phosphorylated form allows researchers to assess MKK4 activation status in models of disease, including cancer, neurodegenerative disorders, and immune responses. Validation typically includes testing in knockout cell lines or phosphorylation-blocking assays to confirm target specificity. This antibody has become a critical tool for dissecting MAPK signaling dynamics in both physiological and pathological contexts.
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