| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB |
| Entrez GeneID | 17395 |
| WB Predicted band size | Calculated MW: 81 kDa; Observed MW: 92 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | Recombinant protein of mouse MMP9 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于MEK2(Ab-394)抗体的3篇模拟参考文献(注：具体文献需根据实际数据库查询，以下为示例模板)：
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1. **Title**: "MEK2 Phosphorylation at Serine 394 Regulates ERK Pathway Activity in Colorectal Cancer"
**Author**: Smith J, et al.
**Summary**: 本研究利用MEK2(Ab-394)抗体检测了结直肠癌细胞中MEK2在Ser394位点的磷酸化水平，发现其磷酸化状态与ERK信号通路的异常激活及肿瘤侵袭性相关，为靶向治疗提供了潜在标志物。
2. **Title**: "Role of MEK2 in Cardiac Hypertrophy: Insights from a Mouse Model"
**Author**: Lee H, et al.
**Summary**: 通过Western blot和免疫组化实验(使用MEK2(Ab-394)抗体)，研究发现MEK2在压力负荷诱导的小鼠心肌肥厚中表达上调，并通过调控ERK1/2影响心肌细胞增殖与纤维化进程。
3. **Title**: "Validation of MEK2-Specific Antibodies for Signaling Pathway Analysis"
**Author**: Garcia R, et al.
**Summary**: 本文系统评估了多种MEK2抗体(包括Ab-394)的特异性与灵敏度，证实MEK2(Ab-394)在检测内源性MEK2蛋白及区分其磷酸化与非磷酸化形式中表现优异，适用于多种实验模型。
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**注意**：以上文献为示例，实际引用需通过PubMed、Google Scholar等平台以“MEK2 antibody Ab-394”或相关关键词检索真实研究。若需具体文献，建议结合抗体生产商(如CST、Santa Cruz)官网提供的参考文献列表。
The MEK2 (Ab-394) antibody is a widely used tool in molecular biology research for detecting mitogen-activated protein kinase kinase 2 (MEK2), a key serine/threonine kinase in the MAPK/ERK signaling pathway. MEK2. encoded by the *MAP2K2* gene, acts downstream of RAF kinases to phosphorylate and activate extracellular signal-regulated kinases 1 and 2 (ERK1/2), regulating cellular processes like proliferation, differentiation, and apoptosis. Dysregulation of MEK2 is implicated in cancers, developmental disorders, and inflammatory diseases.
The Ab-394 antibody specifically recognizes endogenous MEK2 proteins (∼48 kDa) across human, mouse, and rat species. It is commonly employed in techniques such as Western blotting, immunoprecipitation, and immunofluorescence. Researchers use it to study MEK2 expression, activation states (e.g., phosphorylation at Ser222/226), and interactions within signaling cascades, particularly in cancer models or drug discovery targeting the RAF-MEK-ERK pathway.
Validated for specificity, Ab-394 shows minimal cross-reactivity with MEK1 or other MAPK family members. Its applications span basic research and translational studies, including evaluating MEK/ERK inhibitors in oncology. Commercial suppliers often provide detailed validation data, including reactivity in knockout controls. As MEK2 gains attention in precision medicine, this antibody remains a critical reagent for dissecting pathway dynamics in health and disease.
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