| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Megakaryocyte-associated tyrosine-protein kinase, Protein kinase NTK, Tyrosine-protein kinase CTK, Matk, Ctk, Ntk |
| Entrez GeneID | 17179 |
| WB Predicted band size | 56.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Matk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 477-505 amino acids from the C-terminal region of mouse Matk. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PDK1(Ab-241)抗体的示例参考文献(注：部分内容为假设性概括，实际文献需通过学术数据库验证)：
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1. **文献名称**：*Role of PDK1 in Akt/mTOR Signaling and Cancer Cell Survival*
**作者**：Smith J, et al.
**摘要**：本研究利用PDK1(Ab-241)抗体通过Western blot和免疫组化分析，发现PDK1在乳腺癌中高表达，并通过调控Akt磷酸化促进肿瘤细胞存活。抗体特异性验证显示其对PDK1的241位点具有高亲和力。
2. **文献名称**：*PDK1 Phosphorylation at Ser241 Modulates Insulin Signaling in Metabolic Disorders*
**作者**：Lee H, et al.
**摘要**：通过PDK1(Ab-241)抗体的免疫沉淀实验，研究者证实PDK1在Ser241位点的磷酸化是胰岛素信号通路的关键调控步骤，并发现其在Ⅱ型糖尿病模型中的异常激活。
3. **文献名称**：*Validation of PDK1 Antibody Specificity in Neuronal Development*
**作者**：Garcia R, et al.
**摘要**：该研究系统验证了PDK1(Ab-241)抗体在神经元组织中的特异性，通过siRNA敲低和质谱分析确认其识别PDK1的C端结构域，并揭示PDK1在神经突触形成中的作用。
4. **文献名称**：*PDK1 Inhibition Sensitizes Lung Cancer to Chemotherapy*
**作者**：Wang Y, et al.
**摘要**：使用PDK1(Ab-241)进行免疫荧光染色，发现PDK1在非小细胞肺癌中定位在线粒体膜上，其表达水平与化疗耐药性相关，靶向PDK1可增强顺铂疗效。
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**说明**：
- 以上文献为示例，实际引用需通过PubMed、Google Scholar等平台搜索关键词“PDK1 antibody Ab-241”或结合抗体生产商(如CST、Abcam)提供的引用文献列表。
- 若需具体文献，建议访问学术数据库并筛选应用该抗体的实验研究(如Western blot、IP等)。
The PDK1 (Ab-241) antibody is a monoclonal antibody specifically designed to detect 3-phosphoinositide-dependent kinase 1 (PDK1), a serine/threonine kinase critical in regulating cell survival, growth, and metabolism. PDK1 acts as a master regulator of the AGC kinase family, including Akt/PKB, PKC, and SGK, by phosphorylating their activation loops. It is activated downstream of PI3K signaling, linking receptor tyrosine kinase and insulin signaling to downstream metabolic and proliferative pathways. Dysregulation of PDK1 is implicated in cancer, diabetes, and cardiovascular diseases, making it a key therapeutic target.
The Ab-241 clone recognizes an epitope within the N-terminal region of human PDK1. typically detecting a ~63 kDa protein. It is commonly used in applications like Western blotting, immunoprecipitation, and immunofluorescence to study PDK1 expression, localization, and interaction partners in cell lines or tissue samples. Validated for specificity, it shows minimal cross-reactivity with related kinases. Researchers utilize this antibody to explore PDK1's role in signaling pathways, drug response, and disease mechanisms, particularly in contexts involving PI3K/Akt/mTOR pathway activation. Its reliability in diverse experimental models supports its utility in both basic research and translational studies.
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