| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tyrosine-protein kinase CSK, C-Src kinase, Protein-tyrosine kinase MPK-2, p50CSK, Csk |
| Entrez GeneID | 12988 |
| WB Predicted band size | 50.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Mouse Csk antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 67-101amino acids from the N-terminal region of human Mouse Csk. |
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以下是关于Elk-1 (Ab-383)抗体的3篇假设参考文献的简单列举(注：文献为虚构示例，仅作格式参考)：
1. **文献名称**：*Elk-1 Phosphorylation Dynamics in MAPK Signaling Pathways*
**作者**：Chen L, et al.
**摘要**：通过Western blot和免疫荧光实验(使用Elk-1 Ab-383抗体)，研究显示生长因子刺激后Elk-1的磷酸化水平显著升高，调控下游靶基因表达。
2. **文献名称**：*Elk-1/SRF Complex in Cardiac Hypertrophy*
**作者**：Müller S, et al.
**摘要**：采用Elk-1 (Ab-383)抗体进行染色质免疫沉淀(ChIP)，发现Elk-1与血清反应因子(SRF)结合，协同激活心肌细胞肥大相关基因。
3. **文献名称**：*Validation of Elk-1 Antibody Specificity in Neuronal Models*
**作者**：Park J, et al.
**摘要**：通过siRNA敲低实验和肽段阻断验证Elk-1 Ab-383抗体的特异性，证实其适用于检测脑组织中的Elk-1蛋白表达及定位。
4. **文献名称**：*Elk-1 in Oxidative Stress-Induced Apoptosis*
**作者**：Garcia R, et al.
**摘要**：利用Elk-1 Ab-383抗体进行免疫组化分析，揭示氧化应激下Elk-1核易位增强，促进促凋亡基因转录。
(提示：实际文献需通过PubMed或抗体供应商官网的引用列表查询。)
Elk-1 (Ab-383) is an antibody targeting the transcription factor Elk-1. a member of the ETS (E26 transformation-specific) family. Elk-1 plays a critical role in regulating gene expression by binding to specific DNA sequences, often in conjunction with serum response factor (SRF), to activate transcription in response to extracellular signals such as growth factors or stress. It is phosphorylated by mitogen-activated protein kinases (MAPKs) like ERK, JNK, and p38 at specific serine residues (e.g., Ser383 and Ser389), which enhances its transcriptional activity.
The Elk-1 (Ab-383) antibody is commonly used to detect endogenous levels of Elk-1. particularly its phosphorylated form at Ser383. a key modification linked to MAPK pathway activation. This antibody is valuable in studies investigating cellular signaling pathways, neuronal development, apoptosis, and oncogenesis. It is often applied in techniques like Western blotting, immunoprecipitation, and immunofluorescence, with reactivity observed in human, mouse, and rat samples.
Raised in host species like rabbit or mouse, Elk-1 (Ab-383) offers specificity for the phosphorylated epitope, enabling researchers to dissect Elk-1’s role in dynamic processes such as immediate-early gene induction. Proper storage (typically at -20°C) and validation in relevant experimental models are recommended to ensure optimal performance. Its utility extends to both basic research and disease mechanism studies, particularly in cancer and neurological disorders.
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