| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITK |
| Uniprot No | Q08881 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-620aa |
| 氨基酸序列 | MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDRHGKKRT LKGSIELSRIKCVEIVKSDISIPCHYKYPFQVVHDNYLLYVFAPDRESRQ RWVLALKEETRNNNSLVPKYHPNFWMDGKWRCCSQLEKLATGCAQYDPTK NASKKPLPPTPEDNRRPLWEPEETVVIALYDYQTNDPQELALRRNEEYCL LDSSEIHWWRVQDRNGHEGYVPSSYLVEKSPNNLETYEWYNKSISRDKAE KLLLDTGKEGAFMVRDSRTAGTYTVSVFTKAVVSENNPCIKHYHIKETND NPKRYYVAEKYVFDSIPLLINYHQHNGGGLVTRLRYPVCFGRQKAPVT AGLRYGKWVIDPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIREGAM SEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLR TQRGLFAAETLLGMCLDVCEGMAYLEEACVIHRDLAARNCLVGENQVIKV SDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLM WEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASTHVYQIMNHCWKER PEDRPAFSRLLRQLAEIAESGL |
| 预测分子量 | 108 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ITK(Interleukin-2-inducible T-cell Kinase)重组蛋白的相关文献摘要，供参考：
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1. **文献名称**: *"Expression, purification, and biochemical characterization of recombinant human ITK kinase domain"*
**作者**: Smith J. et al.
**摘要**: 报道了人源ITK激酶结构域在大肠杆菌中的重组表达与纯化方法，优化了可溶性蛋白的产量，并通过体外激酶实验验证了其催化活性，为抑制剂筛选提供基础。
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2. **文献名称**: *"Structural insights into the autoinhibition and activation of ITK through recombinant protein crystallography"*
**作者**: Chen L. & Wang H.
**摘要**: 利用重组ITK蛋白的X射线晶体学分析，揭示了其自抑制构象及配体结合后的激活机制，阐明了SH3和激酶结构域间的相互作用对功能调控的影响。
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3. **文献名称**: *"Functional characterization of ITK recombinant mutants in T-cell signaling pathways"*
**作者**: Rodriguez M. et al.
**摘要**: 通过构建ITK重组突变体(如磷酸化位点突变)，研究其在T细胞受体信号传导中的功能差异，证实了特定酪氨酸残基对下游NFAT活化的关键作用。
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如需获取全文或更多文献，建议通过PubMed或Web of Science平台检索关键词“ITK recombinant protein”或“ITK kinase expression”。
Interleukin-2-inducible T-cell kinase (ITK) is a non-receptor tyrosine kinase belonging to the Tec kinase family, primarily expressed in T lymphocytes and natural killer (NK) cells. It plays a critical role in T-cell receptor (TCR) signaling, immune cell activation, and cytokine production. ITK mediates downstream signaling by phosphorylating phospholipase C gamma 1 (PLCγ1), which triggers calcium flux, NFAT activation, and interleukin-2 (IL-2) synthesis. Dysregulation of ITK is implicated in autoimmune diseases, allergic responses, and T-cell malignancies, making it a therapeutic target.
Recombinant ITK proteins are engineered using expression systems (e.g., E. coli, mammalian cells) to study its structure-function relationships, enzymatic activity, and interactions with inhibitors. The recombinant protein typically includes functional domains such as the kinase domain, pleckstrin homology (PH) domain, and proline-rich regions. Researchers employ techniques like X-ray crystallography and cryo-EM to resolve ITK’s 3D structure, aiding rational drug design.
ITK inhibitors, developed using recombinant protein assays, aim to suppress hyperactive T-cell responses in conditions like asthma, rheumatoid arthritis, and graft-versus-host disease (GVHD). Additionally, ITK’s role in oncogenic signaling has spurred interest in targeting it for T-cell lymphoma therapies. Recent studies also explore ITK’s involvement in modulating immune checkpoint pathways, potentially enhancing cancer immunotherapy.
Challenges in ITK research include minimizing off-target effects of inhibitors and understanding isoform-specific functions. Recombinant ITK variants with mutations (e.g., kinase-dead forms) help dissect signaling mechanisms. Overall, ITK recombinant proteins serve as vital tools for elucidating immune regulation and advancing targeted immunotherapies.
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