| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | Serpinb6b |
| Uniprot No | O08804 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-377aa |
| 氨基酸序列 | MDPLLEANATFALNLLKTLGEDSSRNVLFSPISVSSALAMVFMGAKGTTASQMAQALSLDKCSGKGGRDVHQGFQSLLTETNKTGTQYVLRTANRLFGEKTFDILASFKDSCRKFYEAEMEELDFKGATEQSRQHINAWVAKKTEDKITELLSSGSVNSNTPLVLVNAIYFKGNWEKQFNKEDTQEMPFNVTKDVVKPVQMMFQKSTFKMTYVEEISTNILLLPYVGNELNMIIMLPDEHIELSMVEKEITYKKFIEWTRLDKMEEEEVEVFLPKFKLEENYDMKDVLCRLGMTDAFEEGMADFSGIASKEGLFLSKVIHKSFVEVNEEGTEAAAATAANIGFRCMVPYFCANHPFLFFIQHSRTSGIVFCGRFSSP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Serpinb6b重组蛋白的3篇文献示例(内容基于常见研究方向虚构，仅供参考格式)：
1. **文献名称**：*Recombinant Serpinb6b inhibits neutrophil elastase and modulates inflammation in murine models*
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化重组Serpinb6b蛋白，验证其对中性粒细胞弹性蛋白酶的抑制作用，并证明其在减轻小鼠肺部炎症模型中的潜在治疗作用。
2. **文献名称**：*Structural characterization of human Serpinb6b and its interaction with proteases*
**作者**：Lee J, et al.
**摘要**：通过X射线晶体学解析Serpinb6b重组蛋白的三维结构，揭示其与靶标蛋白酶(如胰蛋白酶)的结合机制，为设计抗蛋白酶药物提供结构基础。
3. **文献名称**：*Serpinb6b deficiency leads to hearing loss via dysregulation of cochlear proteostasis*
**作者**：Wang Y, et al.
**摘要**：利用重组Serpinb6b蛋白进行体外功能补偿实验，发现其通过调控耳蜗内蛋白酶平衡维持听觉细胞稳态，缺陷可能导致遗传性耳聋。
如需真实文献，建议通过PubMed或Google Scholar以关键词“Serpinb6b recombinant protein”检索。
Serpinb6b, a member of the serine protease inhibitor (serpin) superfamily, is a protein encoded by the SERPINB6B gene in mice. Serpins are broadly involved in regulating protease-mediated processes such as inflammation, apoptosis, and cellular homeostasis. Serpinb6b, specifically, is an intracellular inhibitor known to target proteases like cathepsin G and chymotrypsin, which are implicated in immune responses and tissue remodeling. Its human ortholog, SERPINB6. shares functional similarities and is associated with diverse physiological roles, including protection against lysosomal protease leakage and modulation of innate immunity.
Recombinant Serpinb6b protein is produced using expression systems like *E. coli* or mammalian cells, enabling large-scale purification for experimental use. This engineered protein retains the inhibitory activity of its native counterpart, often validated through enzymatic assays. Researchers employ recombinant Serpinb6b to study protease-inhibitor interactions, cellular stress responses, and pathways linked to diseases such as cancer, neurodegenerative disorders, or chronic inflammation. Its role in mitigating protease-driven damage makes it relevant in models of ischemia-reperfusion injury or autoimmune conditions.
Additionally, Serpinb6b has been explored for its tissue-specific expression patterns, particularly in barrier tissues (e.g., skin, lung) and immune cells, suggesting localized regulatory functions. Studies using knockout mice highlight its importance in maintaining cellular integrity and preventing uncontrolled proteolysis. As a research tool, recombinant Serpinb6b aids in elucidating serpin mechanisms, developing diagnostic biomarkers, or screening therapeutic compounds targeting protease dysregulation. Ongoing research continues to uncover its potential in precision medicine and biotechnological applications.
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