| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | C8g |
| Uniprot No | P07360 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-202aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMQKPQRPRRPASPISTIQPKANFDAQQFAG TWLLVAVGSACRFLQEQGHRAEATTLHVAPQGTAMAVSTFRKLDGICWQV RQLYGDTGVLGRFLLQARGARGAVHVVVAETDYQSFAVLYLERAGQLSVK LYARSLPVSDSVLSGFEQRVQEAHLTEDQIFYFPKYGFCEAADQFHVLDE VRR |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟的关于C8g重组蛋白的参考文献示例(注：实际文献需通过学术数据库查询)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant Human Complement C8γ in vitro*
**作者**：Smith, J., et al.
**摘要**：本研究成功在大肠杆菌中表达了重组人C8γ蛋白，并验证其在补体膜攻击复合物(MAC)形成中的功能，揭示了其与C8α-β亚基的相互作用机制。
2. **文献名称**：*Structural Insights into C8γ Subunit via Recombinant Protein Crystallography*
**作者**：Zhang, L., et al.
**摘要**：通过重组C8γ蛋白的结晶和结构解析，揭示了其三维构象及与脂质结合的关键区域，为补体系统相关疾病的药物设计提供依据。
3. **文献名称**：*Optimization of C8g Recombinant Production in Mammalian Cell Systems*
**作者**：Brown, K., et al.
**摘要**：在HEK293细胞中优化C8g重组蛋白的表达条件，获得高产量且具有生物活性的蛋白，适用于免疫学研究和治疗性抗体开发。
4. **文献名称**：*Role of Recombinant C8γ in Pathogen Immune Evasion Mechanisms*
**作者**：Garcia, R., et al.
**摘要**：探讨重组C8γ蛋白在细菌感染模型中的作用，发现某些病原体通过干扰C8γ功能抑制MAC组装，从而逃避免疫攻击。
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**提示**：以上为假设性示例，实际文献建议通过PubMed、Web of Science或Google Scholar检索关键词(如“C8γ recombinant protein”“Complement C8 gamma subunit”)获取。
C8γ recombinant protein is a key component in the study of the complement system, a critical part of innate immunity. The complement membrane attack complex (MAC), composed of C5b, C6. C7. C8. and multiple C9 subunits, mediates target cell lysis by forming pores in pathogen membranes. C8. a heterotrimer (α, β, γ), acts as a pivotal bridge in MAC assembly. The C8γ subunit, a 22 kDa protein, belongs to the lipocalin family and is uniquely expressed as a disulfide-linked dimer. Unlike C8α and C8β, which are structurally related to other MAC proteins, C8γ is non-lytic and may modulate MAC function through interactions with lipids or regulatory proteins.
Recombinant C8γ is typically produced using bacterial (e.g., E. coli) or mammalian expression systems, enabling studies on its structural and functional roles. Its recombinant form retains the ability to bind C8α, facilitating research into MAC assembly mechanisms. Researchers employ it to investigate complement-mediated pathologies, including autoimmune disorders, bacterial infections, and inflammatory diseases. Additionally, it serves as a tool for developing inhibitors targeting MAC formation, with therapeutic potential in conditions like paroxysmal nocturnal hemoglobinuria or age-related macular degeneration.
The protein’s production via recombinant technology ensures high purity and reproducibility, addressing challenges in isolating native C8γ from biological sources. Recent studies also explore its non-canonical roles in cell signaling and tissue homeostasis, expanding its relevance beyond traditional complement pathways.
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