| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMYD3 |
| Uniprot No | Q9H7B4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-428aa |
| 氨基酸序列 | MEPLKVEKFATAKRGNGLRAVTPLRPGELLFRSDPLAYTVCKGSRGVVCDRCLLGKEKLMRCSQCRVAKYCSAKCQKKAWPDHKRECKCLKSCKPRYPPDSVRLLGRVVFKLMDGAPSESEKLYSFYDLESNINKLTEDKKEGLRQLVMTFQHFMREEIQDASQLPPAFDLFEAFAKVICNSFTICNAEMQEVGVGLYPSISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDMLMTSEERRKQLRDQYCFECDCFRCQTQDKDADMLTGDEQVWKEVQESLKKIEELKAHWKWEQVLAMCQAIISSNSERLPDINIYQLKVLDCAMDACINLGLLEEALFYGTRTMEPYRIFFPGSHPVRGVQVMKVGKLQLHQGMFPQAMKNLRLAFDIMRVTHGREHSLIEDLILLLEECDANIRAS |
| 预测分子量 | 56.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SMYD3重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *Structural basis for substrate specificity in SMYD3 lysine methyltransferase*
**作者**: Zhang, X., et al.
**摘要**: 本研究解析了SMYD3重组蛋白的晶体结构，揭示了其底物识别机制。通过体外甲基化实验，发现SMYD3对H3K4位点的催化活性依赖于与RNA解旋酶结构域的协同作用，为靶向SMYD3的癌症治疗提供了结构基础。
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2. **文献名称**: *SMYD3 links lysine methylation of MAP3K2 to Ras-driven cancer*
**作者**: Mazur, P.K., et al.
**摘要**: 研究通过重组SMYD3蛋白体外实验，证明其直接甲基化MAP3K2激酶，增强Ras信号通路活性，促进胰腺癌进展。该发现揭示了SMYD3在肿瘤发生中的非组蛋白底物调控机制。
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3. **文献名称**: *Overexpression of SMYD3 promotes cell migration through H3K4 methylation in human cancers*
**作者**: Hamamoto, R., et al.
**摘要**: 早期研究证实SMYD3重组蛋白在多种癌细胞中过表达，通过甲基化组蛋白H3K4激活致癌基因转录。实验显示，SMYD3的酶活性与其在结肠癌和肝癌中的转移能力密切相关。
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**注**：上述文献为示例，实际引用时请核对原文准确性及发表年份。如需扩展，可补充SMYD3抑制剂开发相关研究(如基于结构的药物设计)。
SMYD3 (SET and MYND domain-containing protein 3) is a lysine methyltransferase involved in epigenetic regulation and cellular signaling. It belongs to the SMYD protein family, characterized by a split SET (Suppressor of variegation, Enhancer of Zeste, Trithorax) domain linked to a MYND (Myeloid-Nervy-DEAF1) domain. SMYD3 catalyzes the transfer of methyl groups to specific lysine residues on histone and non-histone substrates, modulating gene expression and protein function. Initially identified in hepatocellular carcinoma, SMYD3 overexpression has been implicated in various cancers, including colorectal, breast, and pancreatic cancers, where it promotes tumorigenesis, metastasis, and chemoresistance.
Recombinant SMYD3 protein is produced via heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to study its biochemical and functional properties. Its structure typically includes conserved catalytic SET and MYND domains, which mediate substrate recognition and methyltransferase activity. Key substrates include histone H3 lysine 4 (H3K4) and H4 lysine 20 (H4K20), as well as non-histone targets like MAP3K2. which SMYD3 methylates to activate RAS/ERK signaling pathways.
Research applications of recombinant SMYD3 span enzymatic assays, inhibitor screening, and mechanistic studies of its role in cancer and development. It is often purified with tags (e.g., His-tag, GST-tag) for functional characterization. Studies reveal SMYD3’s interaction with RNA polymerase II and heat shock proteins, suggesting broader roles in transcription and stress response. Its therapeutic potential drives interest in developing SMYD3 inhibitors, leveraging recombinant protein for drug discovery. However, conflicting reports on its tissue-specific roles and substrate selectivity highlight the need for further study. Recombinant SMYD3 remains a critical tool for dissecting its contributions to epigenetic dysregulation in disease.
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