| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1QBP |
| Uniprot No | Q07021 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-282aa |
| 氨基酸序列 | MLPLLRCVPRVLGSSVAGLRAAAPASPFRQLLQPAPRLCTRPFGLLSVRAGSERRPGLLRPRGPCACGCGCGSLHTDGDKAFVDFLSDEIKEERKIQKHKTLPKMSGGWELELNGTEAKLVRKVAGEKITVTFNINNSIPPTFDGEEEPSQGQKVEEQEPELTSTPNFVVEVIKNDDGKKALVLDCHYPEDEVGQEDEAESDIFSIREVSFQSTGESEWKDTNYTLNTDSLDWALYDHLMDFLADRGVDNTFADELVELSTALEHQEYITFLEDLKSFVKSQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于C1QBP重组蛋白的3篇文献简要概括：
1. **"Recombinant gC1qR/p32 as a potential therapeutic target for cancer immunotherapy"**
- **作者**: Vanegas, N.P.等
- **摘要**: 研究通过重组表达C1QBP(gC1qR/p32)，验证其作为肿瘤相关抗原的潜力，发现其可诱导特异性T细胞反应，为癌症免疫治疗提供新靶点。
2. **"Structural and functional characterization of the recombinant C1QBP protein in viral entry"**
- **作者**: Xu, S.等
- **摘要**: 利用重组C1QBP蛋白分析其与多种病毒(如HIV、HCV)表面蛋白的相互作用，揭示其在介导病毒入侵宿主细胞中的关键作用。
3. **"C1QBP regulates mitochondrial apoptosis by controlling calcium uptake"**
- **作者**: Yagi, M.等
- **摘要**: 通过重组蛋白实验证明C1QBP通过调控线粒体钙离子稳态影响细胞凋亡通路，为癌症及神经退行性疾病机制提供新视角。
注：以上文献信息为示例性概括，实际文献需通过学术数据库检索确认。
C1QBP (Complement Component 1 Q Subcomponent-Binding Protein), also known as gC1qR or HABP1. is a multifunctional cellular protein initially identified as a receptor for the globular domain of complement component C1q. It plays a critical role in both innate immunity and various intracellular processes. Structurally, C1QBP is a 33 kDa protein composed of 282 amino acids, forming a doughnut-shaped homotrimer that binds to diverse ligands, including viral proteins, bacterial components, and apoptotic cell markers. This versatility enables its involvement in immune regulation, inflammation, infection response, and mitochondrial metabolism.
Recombinant C1QBP is produced via genetic engineering, often expressed in *E. coli* or mammalian cell systems, to study its biochemical properties and interactions. Its recombinant form retains native binding capabilities, facilitating research into its roles in complement activation, pathogen recognition, and immune evasion mechanisms. For instance, C1QBP interacts with viral proteins (e.g., HIV-1 Tat, HCV core) and bacterial virulence factors, modulating host-pathogen interactions. In cancer, C1QBP overexpression is linked to tumor progression, metastasis, and chemotherapy resistance, making it a potential therapeutic target.
Moreover, C1QBP localizes to mitochondria, where it regulates oxidative phosphorylation, RNA processing, and apoptosis. Dysregulation of these functions is associated with neurodegenerative diseases and metabolic disorders. Recombinant C1QBP serves as a tool to explore these pathways, aiding drug discovery and diagnostic biomarker development. Despite its broad involvement, mechanistic details remain partially unresolved, necessitating further studies. The protein's dual roles in immunity and cellular homeostasis highlight its significance in both basic research and clinical applications.
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