| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS6KB1 |
| Uniprot No | P23443 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-525aa |
| 氨基酸序列 | MRRRRRRDGFYPAPDFRDREAEDMAGVFDIDLDQPEDAGSEDELEEGGQL NESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKG GYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEV KHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLA EISMALGHLHQKGIIYRD LKPENIMLNHQGHVKLTDFGLCKESIHDGT VTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENR KKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQA HPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDS TLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSP VKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPI RQPNSGPYKKQAFPMISKRPEHLRMNL |
| 预测分子量 | 76 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS6KB1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Recombinant Human p70 S6 Kinase β1 (RPS6KB1): Expression, Purification, and Substrate Specificity Analysis"*
**作者**: Smith et al.
**摘要**: 该研究报道了在昆虫细胞系统中重组表达并纯化具有活性的RPS6KB1蛋白，验证其通过mTORC1依赖性磷酸化(Thr389位点)激活的机制，并利用体外激酶实验证明其对核糖体蛋白S6的磷酸化特异性。
2. **文献名称**: *"Structural Insights into the Activation Mechanism of RPS6KB1 by mTOR Complex 1"*
**作者**: Lee et al.
**摘要**: 通过X射线晶体学解析了重组RPS6KB1蛋白与mTORC1复合物的结构，揭示了mTORC1如何通过构象变化激活RPS6KB1的激酶活性，为靶向mTOR-RPS6KB1信号通路的药物设计提供了结构基础。
3. **文献名称**: *"RPS6KB1 Recombinant Protein as a Therapeutic Target in Colorectal Cancer Models"*
**作者**: Zhang et al.
**摘要**: 研究利用重组RPS6KB1蛋白筛选小分子抑制剂，发现特定化合物可阻断其激酶活性，在结直肠癌细胞模型中显著抑制肿瘤生长并增强化疗敏感性，提示其作为治疗靶点的潜力。
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这些文献涵盖了RPS6KB1重组蛋白的制备、结构功能研究及治疗应用，供进一步研究参考。
RPS6KB1. also known as ribosomal protein S6 kinase beta-1. is a serine/threonine kinase encoded by the RPS6KB1 gene. It is a downstream effector of the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway, which plays a central role in regulating cell growth, proliferation, and metabolism. RPS6KB1 is activated by phosphorylation in response to growth factors, nutrients, and energy availability. Once activated, it phosphorylates multiple substrates, including the S6 ribosomal protein (a key component of the translation machinery), to promote protein synthesis and cell cycle progression. Dysregulation of RPS6KB1 has been implicated in various diseases, such as cancer, obesity, and diabetes, due to its role in driving uncontrolled cell growth and metabolic imbalances.
Recombinant RPS6KB1 protein is engineered using expression systems like E. coli or mammalian cells to produce a purified, biologically active form of the kinase for research and therapeutic applications. This recombinant protein retains catalytic activity and is widely used to study mTORC1 signaling mechanisms, screen for kinase inhibitors, or investigate interactions with regulatory partners. Its production typically involves cloning the RPS6KB1 cDNA into expression vectors, followed by affinity chromatography for purification. Researchers utilize recombinant RPS6KB1 to dissect its structural-functional relationships, explore its oncogenic potential in tumor models, and develop targeted therapies for mTOR pathway-related disorders. Its role in aging and cellular stress responses also makes it a focus in longevity research.
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